Effects of carbonyl cyanide phenylhydrazones on two mitochondrial ion channel activities

Maria Luisa Campo, Henry Tedeschi, Concepción Muro, Kathleen W. Kinnally

Research output: Contribution to journalArticle

Abstract

The respiratory uncouplers carbonyl cyanide p- trifluommethoxyphenylhydrazone (FCCP) and carbonyl cyanide m- chlurophenylhydrazone (CCCP) affect the activities of two mitochondrial ion channels from mouse liven. At micromolar concentrations, the phenylhydrazones block the voltage-dependent 100-pS channel, mCS, and induce the multiple- conductance-level channel, MCC. The binding site(s) involved in perturbation of channel activities are probably distinct from the sings involved in uncoupling of oxidative phosphorylation which occurs at nanomolar concentrations of the phenylhydrazones. The effects of FCCP and CCCP on the mitochondrial ion channels could be partially reversed by washing with fresh media and were always reversed by perfusion with dithiothreitol. These results indicate that the effects of the phenylhydrazones on mitochondrial ion channels may be related to the ability of these compounds to act as sulfhydryl reagents and not to their protonophoric and uncoupling activity.

Original languageEnglish (US)
Pages (from-to)223-231
Number of pages9
JournalJournal of Bioenergetics and Biomembranes
Volume29
Issue number3
DOIs
StatePublished - 1997

Keywords

  • Channels
  • MCC
  • Mitochondria
  • Patch-clamp
  • Uncouplers
  • mCS

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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