Effects of different re-wetting techniques on dentin shear bond strengths

André V. Ritter, Harald O. Heymann, Edward J. Swift, Jorge PerdigÃo, Bruno T. Rosa

Research output: Contribution to journalArticlepeer-review


Purpose: For contemporary hydrophilic resin adhesive systems, bonding to dentin is improved if the substrate is maintained in a hydrated state following acid-etching. The purpose of this study was to compare the dentin shear bond strengths of two single-bottle adhesives (one acetone-based and one ethanol-based) applied under different etched-dentin conditions: dry, wet, or dry and re-wetted with different solutions. Materials and Methods: Bovine incisors (N = 120) were mounted in acrylic, polished to 600-grit, and randomly assigned to 12 groups (n = 10). Dentin was etched for 15 seconds using 35% phosphoric acid, rinsed, and either blot-dried, air-dried, or air-dried and re-wetted with different solutions (distilled water, Gluma Desensitizer, Aqua-Prep, and 5% glutaraldehyde in water). Two adhesives (Single Bond and Prime & Bond NT) were applied to each of the surface conditions following manufacturers’ instructions. After adhesive application and curing, composite was applied in a No. 5 gelatin capsule and light-cured. Specimens were loaded in shear, using an Instron at 5 mm per minute. Shear bond strengths were calculated by dividing the failure load by the bonded surface area. Data were subjected to analysis of variance (ANOVA) and a post hoc Tukey test. Results: Mean shear bond strengths ranged from 12.5 to 26.6 MPa for Single Bond and from 5.6 to 14.7 MPa for Prime & Bond NT. Significant differences were found in both groups of materials (p <.001). The three highest mean bond strengths were obtained (in order) on dentin that was re-wetted with Gluma Desensitizer, re-wetted with Aqua-Prep, or never dried. Differences between these surface conditions were not statistically significant for either material. CLINICAL SIGNIFICANCE. Different dentin surface conditions and re-wetting techniques affected bond strengths for adhesives studied. Aqua-Prep and Gluma Desensitizer can be successfully used as re-wetting agents. The use of a re-wetting agent may be beneficial when dentin is dried after acid-etching and rinsing.

Original languageEnglish (US)
Pages (from-to)85-96
Number of pages12
JournalJournal of Esthetic and Restorative Dentistry
Issue number2
StatePublished - Mar 2000

ASJC Scopus subject areas

  • General Dentistry


Dive into the research topics of 'Effects of different re-wetting techniques on dentin shear bond strengths'. Together they form a unique fingerprint.

Cite this