Effects of phosphoric acid and glutaraldehyde-HEMA on dentin collagen

André V. Ritter, Edward J. Swift, Mitsuo Yamauchi

Research output: Contribution to journalArticlepeer-review


The purpose of this study was to evaluate the effects of phosphoric acid (PA) and a proprietary glutaraldehyde-HEMA aqueous solution (Gluma Dcsensitizer; GD) on dentin collagen. Specimens of demineralized bovine dentin collagen were treated with either 37% or 50% PA for 1 or 5 min. An additional set of specimens was treated with 37% PA for 1 min followed by GD for 1 min. All specimens were washed with distilled water, lyophilized, reduced with standardized NaB3H4, hydrolyzed with 6 M HCl and subjected to amino acid and cross-link analyses. The results demonstrated that the treatment of demineralized dentin with PA under the conditions tested did not significantly alter the collagen cross-links. The GD-treated samples showed reduction of free lysine (Lys) and hydroxylysine (Hyl) residues, as well as a decrease in the levels of collagen reducible cross-links. In addition, unidentified reducible compounds were detected by high-performance liquid chromatography (HPLC) analysis. These compounds may be derived from cross-links formed between GD-derived aldehyde and Lys/Hyl of collagen. The findings indicate that PA treatment does not significantly affect dentin collagen amino acid and cross-link composition, and that GD treatment affects dentin collagen amino acid and cross-link composition.

Original languageEnglish (US)
Pages (from-to)348-353
Number of pages6
JournalEuropean Journal of Oral Sciences
Issue number5
StatePublished - Oct 2001


  • Collagen
  • Cross-links
  • Dentin bonding
  • Glutaraldehyde
  • Phosphoric acid

ASJC Scopus subject areas

  • General Dentistry


Dive into the research topics of 'Effects of phosphoric acid and glutaraldehyde-HEMA on dentin collagen'. Together they form a unique fingerprint.

Cite this