Efficient in vivo gene delivery using modified Tat peptide with cationic lipids

Seiichi Yamano; Jisen Dai; Shigeru Hanatani; Ken Haku; Takuto Yamanaka; Mika Ishioka; Tadahiro Takayama; Amr M. Moursi

doi:10.1007/s10529-014-1497-2

Efficient in vivo gene delivery using modified Tat peptide with cationic lipids

Seiichi Yamano, Jisen Dai, Shigeru Hanatani, Ken Haku, Takuto Yamanaka, Mika Ishioka, Tadahiro Takayama, Amr M. Moursi

Research output: Contribution to journal › Article › peer-review

Abstract

A combination of modified HIV-1 Tat (mTat) peptide and cationic lipids, FuGENE HD (FH), dramatically enhanced transfection efficiency across a range of cell lines when compared to mTat or FH alone (Biomaterials 35:1705-1715 2014). The efficiency of this Tat peptide combination was significantly higher than many commercial non-viral vectors. In this present study, we tested the feasibility of this non-viral vector, mTat/FH, in vivo using plasmid DNA encoding a luciferase gene. The results of the in vivo studies showed that animals administered mTat/FH/DNA intramuscularly had significantly higher and longer luciferase expression (≈7 months) than those with mTat/DNA, FH/DNA, or DNA alone. Histological evaluation showed little immune response in the muscles, livers, and kidneys of mice administered with the mTat/FH. The combination of mTat with FH could significantly improve transfection efficiency, expanding the potential use of non-viral gene vectors in vivo.

Original language	English (US)
Pages (from-to)	1447-1452
Number of pages	6
Journal	Biotechnology Letters
Volume	36
Issue number	7
DOIs	https://doi.org/10.1007/s10529-014-1497-2
State	Published - Jun 2014

Keywords

Cationic lipids
Cell-penetrating peptides
Gene delivery
Non-viral vector
Plasmid DNA
Tat peptide
Transfection efficiency

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-014-1497-2

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title = "Efficient in vivo gene delivery using modified Tat peptide with cationic lipids",

abstract = "A combination of modified HIV-1 Tat (mTat) peptide and cationic lipids, FuGENE HD (FH), dramatically enhanced transfection efficiency across a range of cell lines when compared to mTat or FH alone (Biomaterials 35:1705-1715 2014). The efficiency of this Tat peptide combination was significantly higher than many commercial non-viral vectors. In this present study, we tested the feasibility of this non-viral vector, mTat/FH, in vivo using plasmid DNA encoding a luciferase gene. The results of the in vivo studies showed that animals administered mTat/FH/DNA intramuscularly had significantly higher and longer luciferase expression (≈7 months) than those with mTat/DNA, FH/DNA, or DNA alone. Histological evaluation showed little immune response in the muscles, livers, and kidneys of mice administered with the mTat/FH. The combination of mTat with FH could significantly improve transfection efficiency, expanding the potential use of non-viral gene vectors in vivo.",

keywords = "Cationic lipids, Cell-penetrating peptides, Gene delivery, Non-viral vector, Plasmid DNA, Tat peptide, Transfection efficiency",

author = "Seiichi Yamano and Jisen Dai and Shigeru Hanatani and Ken Haku and Takuto Yamanaka and Mika Ishioka and Tadahiro Takayama and Moursi, {Amr M.}",

year = "2014",

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doi = "10.1007/s10529-014-1497-2",

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T1 - Efficient in vivo gene delivery using modified Tat peptide with cationic lipids

AU - Yamano, Seiichi

AU - Dai, Jisen

AU - Hanatani, Shigeru

AU - Haku, Ken

AU - Yamanaka, Takuto

AU - Ishioka, Mika

AU - Takayama, Tadahiro

AU - Moursi, Amr M.

PY - 2014/6

Y1 - 2014/6

N2 - A combination of modified HIV-1 Tat (mTat) peptide and cationic lipids, FuGENE HD (FH), dramatically enhanced transfection efficiency across a range of cell lines when compared to mTat or FH alone (Biomaterials 35:1705-1715 2014). The efficiency of this Tat peptide combination was significantly higher than many commercial non-viral vectors. In this present study, we tested the feasibility of this non-viral vector, mTat/FH, in vivo using plasmid DNA encoding a luciferase gene. The results of the in vivo studies showed that animals administered mTat/FH/DNA intramuscularly had significantly higher and longer luciferase expression (≈7 months) than those with mTat/DNA, FH/DNA, or DNA alone. Histological evaluation showed little immune response in the muscles, livers, and kidneys of mice administered with the mTat/FH. The combination of mTat with FH could significantly improve transfection efficiency, expanding the potential use of non-viral gene vectors in vivo.

AB - A combination of modified HIV-1 Tat (mTat) peptide and cationic lipids, FuGENE HD (FH), dramatically enhanced transfection efficiency across a range of cell lines when compared to mTat or FH alone (Biomaterials 35:1705-1715 2014). The efficiency of this Tat peptide combination was significantly higher than many commercial non-viral vectors. In this present study, we tested the feasibility of this non-viral vector, mTat/FH, in vivo using plasmid DNA encoding a luciferase gene. The results of the in vivo studies showed that animals administered mTat/FH/DNA intramuscularly had significantly higher and longer luciferase expression (≈7 months) than those with mTat/DNA, FH/DNA, or DNA alone. Histological evaluation showed little immune response in the muscles, livers, and kidneys of mice administered with the mTat/FH. The combination of mTat with FH could significantly improve transfection efficiency, expanding the potential use of non-viral gene vectors in vivo.

KW - Cationic lipids

KW - Cell-penetrating peptides

KW - Gene delivery

KW - Non-viral vector

KW - Plasmid DNA

KW - Tat peptide

KW - Transfection efficiency

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Efficient in vivo gene delivery using modified Tat peptide with cationic lipids

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Keywords

ASJC Scopus subject areas

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