Efficient in vivo gene delivery using modified Tat peptide with cationic lipids

Seiichi Yamano, Jisen Dai, Shigeru Hanatani, Ken Haku, Takuto Yamanaka, Mika Ishioka, Tadahiro Takayama, Amr M. Moursi

Research output: Contribution to journalArticle

Abstract

A combination of modified HIV-1 Tat (mTat) peptide and cationic lipids, FuGENE HD (FH), dramatically enhanced transfection efficiency across a range of cell lines when compared to mTat or FH alone (Biomaterials 35:1705-1715 2014). The efficiency of this Tat peptide combination was significantly higher than many commercial non-viral vectors. In this present study, we tested the feasibility of this non-viral vector, mTat/FH, in vivo using plasmid DNA encoding a luciferase gene. The results of the in vivo studies showed that animals administered mTat/FH/DNA intramuscularly had significantly higher and longer luciferase expression (≈7 months) than those with mTat/DNA, FH/DNA, or DNA alone. Histological evaluation showed little immune response in the muscles, livers, and kidneys of mice administered with the mTat/FH. The combination of mTat with FH could significantly improve transfection efficiency, expanding the potential use of non-viral gene vectors in vivo.

Original languageEnglish (US)
Pages (from-to)1447-1452
Number of pages6
JournalBiotechnology Letters
Volume36
Issue number7
DOIs
StatePublished - Jun 2014

Keywords

  • Cationic lipids
  • Cell-penetrating peptides
  • Gene delivery
  • Non-viral vector
  • Plasmid DNA
  • Tat peptide
  • Transfection efficiency

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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