Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe

J. B. Keeney, J. D. Boeke

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Homologous integration into the fission yeast Schizosaccharomyces pombe has not been well characterized. In this study, we have examined integration of plasmids carrying the leu1+ and ura4+ genes into their chromosomal loci. Genomic DNA blot analysis demonstrated that the majority of transformants have one or more copies of the plasmid vector integrated via homologous recombination with a much smaller fraction of gene conversion to leu1+ or ura4+. Non-homologous recombination events were not observed for either gene. We describe the construction of generally useful leu1+ and ura4+ plasmids for targeted integration at the leu1-32 and ura4-294 loci of S. pombe.

    Original languageEnglish (US)
    Pages (from-to)849-856
    Number of pages8
    JournalGenetics
    Volume136
    Issue number3
    StatePublished - 1994

    ASJC Scopus subject areas

    • Genetics

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