Electrical silencing of Drosophila pacemaker neurons stops the free-running circadian clock

Michael N. Nitabach, Justin Blau, Todd C. Holmes

Research output: Contribution to journalArticlepeer-review

Abstract

Electrical silencing of Drosophila circadian pacemaker neurons through targeted expression of K+ channels causes severe deficits in free-running circadian locomotor rhythmicity in complete darkness. Pacemaker electrical silencing also stops the free-running oscillation of PERIOD (PER) and TIMELESS (TIM) proteins that constitutes the core of the cell-autonomous molecular clock. In contrast, electrical silencing fails to abolish PER and TIM oscillation in light-dark cycles, although it does impair rhythmic behavior. On the basis of these findings, we propose that electrical activity is an essential element of the free-running molecular clock of pacemaker neurons along with the transcription factors and regulatory enzymes that have been previously identified as required for clock function.

Original languageEnglish (US)
Pages (from-to)485-495
Number of pages11
JournalCell
Volume109
Issue number4
DOIs
StatePublished - May 17 2002

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Electrical silencing of Drosophila pacemaker neurons stops the free-running circadian clock'. Together they form a unique fingerprint.

Cite this