Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection

Smruti Pushalkar; Bidisha Paul; Qianhao Li; Jian Yang; Rebeca Vasconcelos; Shreya Makwana; Juan Muñoz González; Shivm Shah; Chengzhi Xie; Malvin N. Janal; Erica Queiroz; Maria Bederoff; Joshua Leinwand; Julia Solarewicz; Fangxi Xu; Eman Aboseria; Yuqi Guo; Deanna Aguallo; Claudia Gomez; Angela Kamer; Donna Shelley; Yindalon Aphinyanaphongs; Cheryl Barber; Terry Gordon; Patricia Corby; Xin Li; Deepak Saxena

doi:10.1016/j.isci.2020.100884

Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection

Smruti Pushalkar, Bidisha Paul, Qianhao Li, Jian Yang, Rebeca Vasconcelos, Shreya Makwana, Juan Muñoz González, Shivm Shah, Chengzhi Xie, Malvin N. Janal, Erica Queiroz, Maria Bederoff, Joshua Leinwand, Julia Solarewicz, Fangxi Xu, Eman Aboseria, Yuqi Guo, Deanna Aguallo, Claudia Gomez, Angela KamerDonna Shelley, Yindalon Aphinyanaphongs, Cheryl Barber, Terry Gordon, Patricia Corby, Xin Li, Deepak Saxena

Research output: Contribution to journal › Article › peer-review

Abstract

The trend of e-cigarette use among teens is ever increasing. Here we show the dysbiotic oral microbial ecology in e-cigarette users influencing the local host immune environment compared with non-smoker controls and cigarette smokers. Using 16S rRNA high-throughput sequencing, we evaluated 119 human participants, 40 in each of the three cohorts, and found significantly altered beta-diversity in e-cigarette users (p = 0.006) when compared with never smokers or tobacco cigarette smokers. The abundance of Porphyromonas and Veillonella (p = 0.008) was higher among vapers. Interleukin (IL)-6 and IL-1β were highly elevated in e-cigarette users when compared with non-users. Epithelial cell-exposed e-cigarette aerosols were more susceptible for infection. In vitro infection model of premalignant Leuk-1 and malignant cell lines exposed to e-cigarette aerosol and challenged by Porphyromonas gingivalis and Fusobacterium nucleatum resulted in elevated inflammatory response. Our findings for the first time demonstrate that e-cigarette users are more prone to infection.

Original language	English (US)
Article number	100884
Journal	iScience
Volume	23
Issue number	3
DOIs	https://doi.org/10.1016/j.isci.2020.100884
State	Published - Mar 27 2020

Keywords

In Vitro Toxicology
Microbiome
Oral Microbiology

ASJC Scopus subject areas

General

Access to Document

10.1016/j.isci.2020.100884

Cite this

Pushalkar, S., Paul, B., Li, Q., Yang, J., Vasconcelos, R., Makwana, S., González, J. M., Shah, S., Xie, C., Janal, M. N., Queiroz, E., Bederoff, M., Leinwand, J., Solarewicz, J., Xu, F., Aboseria, E., Guo, Y., Aguallo, D., Gomez, C., ... Saxena, D. (2020). Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection. iScience, 23(3), Article 100884. https://doi.org/10.1016/j.isci.2020.100884

Pushalkar, S, Paul, B, Li, Q, Yang, J, Vasconcelos, R, Makwana, S, González, JM, Shah, S, Xie, C, Janal, MN, Queiroz, E, Bederoff, M, Leinwand, J, Solarewicz, J, Xu, F, Aboseria, E, Guo, Y, Aguallo, D, Gomez, C, Kamer, A , Shelley, D, Aphinyanaphongs, Y, Barber, C, Gordon, T, Corby, P, Li, X & Saxena, D 2020, 'Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection', iScience, vol. 23, no. 3, 100884. https://doi.org/10.1016/j.isci.2020.100884

@article{556a13449b9b42c4b494b9d9dbda960b,

title = "Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection",

abstract = "The trend of e-cigarette use among teens is ever increasing. Here we show the dysbiotic oral microbial ecology in e-cigarette users influencing the local host immune environment compared with non-smoker controls and cigarette smokers. Using 16S rRNA high-throughput sequencing, we evaluated 119 human participants, 40 in each of the three cohorts, and found significantly altered beta-diversity in e-cigarette users (p = 0.006) when compared with never smokers or tobacco cigarette smokers. The abundance of Porphyromonas and Veillonella (p = 0.008) was higher among vapers. Interleukin (IL)-6 and IL-1β were highly elevated in e-cigarette users when compared with non-users. Epithelial cell-exposed e-cigarette aerosols were more susceptible for infection. In vitro infection model of premalignant Leuk-1 and malignant cell lines exposed to e-cigarette aerosol and challenged by Porphyromonas gingivalis and Fusobacterium nucleatum resulted in elevated inflammatory response. Our findings for the first time demonstrate that e-cigarette users are more prone to infection.",

keywords = "In Vitro Toxicology, Microbiome, Oral Microbiology",

author = "Smruti Pushalkar and Bidisha Paul and Qianhao Li and Jian Yang and Rebeca Vasconcelos and Shreya Makwana and Gonz{\'a}lez, {Juan Mu{\~n}oz} and Shivm Shah and Chengzhi Xie and Janal, {Malvin N.} and Erica Queiroz and Maria Bederoff and Joshua Leinwand and Julia Solarewicz and Fangxi Xu and Eman Aboseria and Yuqi Guo and Deanna Aguallo and Claudia Gomez and Angela Kamer and Donna Shelley and Yindalon Aphinyanaphongs and Cheryl Barber and Terry Gordon and Patricia Corby and Xin Li and Deepak Saxena",

note = "Publisher Copyright: {\textcopyright} 2020 The Author(s)",

year = "2020",

month = mar,

day = "27",

doi = "10.1016/j.isci.2020.100884",

language = "English (US)",

volume = "23",

journal = "iScience",

issn = "2589-0042",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection

AU - Pushalkar, Smruti

AU - Paul, Bidisha

AU - Li, Qianhao

AU - Yang, Jian

AU - Vasconcelos, Rebeca

AU - Makwana, Shreya

AU - González, Juan Muñoz

AU - Shah, Shivm

AU - Xie, Chengzhi

AU - Janal, Malvin N.

AU - Queiroz, Erica

AU - Bederoff, Maria

AU - Leinwand, Joshua

AU - Solarewicz, Julia

AU - Xu, Fangxi

AU - Aboseria, Eman

AU - Guo, Yuqi

AU - Aguallo, Deanna

AU - Gomez, Claudia

AU - Kamer, Angela

AU - Shelley, Donna

AU - Aphinyanaphongs, Yindalon

AU - Barber, Cheryl

AU - Gordon, Terry

AU - Corby, Patricia

AU - Li, Xin

AU - Saxena, Deepak

PY - 2020/3/27

Y1 - 2020/3/27

N2 - The trend of e-cigarette use among teens is ever increasing. Here we show the dysbiotic oral microbial ecology in e-cigarette users influencing the local host immune environment compared with non-smoker controls and cigarette smokers. Using 16S rRNA high-throughput sequencing, we evaluated 119 human participants, 40 in each of the three cohorts, and found significantly altered beta-diversity in e-cigarette users (p = 0.006) when compared with never smokers or tobacco cigarette smokers. The abundance of Porphyromonas and Veillonella (p = 0.008) was higher among vapers. Interleukin (IL)-6 and IL-1β were highly elevated in e-cigarette users when compared with non-users. Epithelial cell-exposed e-cigarette aerosols were more susceptible for infection. In vitro infection model of premalignant Leuk-1 and malignant cell lines exposed to e-cigarette aerosol and challenged by Porphyromonas gingivalis and Fusobacterium nucleatum resulted in elevated inflammatory response. Our findings for the first time demonstrate that e-cigarette users are more prone to infection.

AB - The trend of e-cigarette use among teens is ever increasing. Here we show the dysbiotic oral microbial ecology in e-cigarette users influencing the local host immune environment compared with non-smoker controls and cigarette smokers. Using 16S rRNA high-throughput sequencing, we evaluated 119 human participants, 40 in each of the three cohorts, and found significantly altered beta-diversity in e-cigarette users (p = 0.006) when compared with never smokers or tobacco cigarette smokers. The abundance of Porphyromonas and Veillonella (p = 0.008) was higher among vapers. Interleukin (IL)-6 and IL-1β were highly elevated in e-cigarette users when compared with non-users. Epithelial cell-exposed e-cigarette aerosols were more susceptible for infection. In vitro infection model of premalignant Leuk-1 and malignant cell lines exposed to e-cigarette aerosol and challenged by Porphyromonas gingivalis and Fusobacterium nucleatum resulted in elevated inflammatory response. Our findings for the first time demonstrate that e-cigarette users are more prone to infection.

KW - In Vitro Toxicology

KW - Microbiome

KW - Oral Microbiology

UR - http://www.scopus.com/inward/record.url?scp=85082183738&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85082183738&partnerID=8YFLogxK

U2 - 10.1016/j.isci.2020.100884

DO - 10.1016/j.isci.2020.100884

M3 - Article

C2 - 32105635

AN - SCOPUS:85082183738

SN - 2589-0042

VL - 23

JO - iScience

JF - iScience

IS - 3

M1 - 100884

ER -

Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this