Endothelial Signals Modulate Hepatocyte Apicobasal Polarization in Zebrafish

Takuya F. Sakaguchi, Kirsten C. Sadler, Cecile Crosnier, Didier Y.R. Stainier

Research output: Contribution to journalArticlepeer-review


Emerging evidence indicates that paracrine signals from endothelial cells play a role in tissue differentiation and organ formation [1-3]. Here, we identify a novel role for endothelial cells in modulating hepatocyte polarization during liver organogenesis. We find that in zebrafish, the apical domain of the hepatocytes predicts the location of the intrahepatic biliary network. The refinement of hepatocyte polarization coincides with the invasion of endothelial cells into the liver, and these endothelial cells migrate along the maturing basal surface of the hepatocytes. Using genetic, pharmacological, and transplantation experiments, we provide evidence that endothelial cells influence the polarization of the adjacent hepatocytes. This influence of endothelial cells on hepatocytes is mediated at least in part by the cell-surface protein Heart of glass and contributes to the establishment of coordinately aligned hepatocyte apical membranes and evenly spaced intrahepatic conduits.

Original languageEnglish (US)
Pages (from-to)1565-1571
Number of pages7
JournalCurrent Biology
Issue number20
StatePublished - Oct 28 2008



ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


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