Enhanced synaptophysin immunoreactivity in rat hippocampal culture by 5-HT1A agonist, S100b, and corticosteroid receptor agonists

Mayumi Nishi, Patricia M. Whitaker-Azmitia, Efrain C. Azmitia

Research output: Contribution to journalArticlepeer-review


Serotonin (5-HT) has been shown to modulate brain maturation during development and adult plasticity. This effect in the whole animal may be due to activation of 5-HT1A receptors and a corresponding increases in S100b and corticosterone. Synaptophysin, an integral protein of the synaptic vesicle membrane that correlates with synaptic density and neurotransmitter release, is reduced by depletion of 5-HT in the cortex and hippocampus of the adult rat. Injections of a 5-HT1A agonist or dexamethasone can reverse the loss of synaptophysin immunoreactivity (IR). In this study we used morphometric analysis of synaptophysin-IR to study the effects of the 5-HT1A agonist, ipsapirone, and the neuronal extension factor, S100b on hippocampal neurons grown in a serum and steroid free media. Both compounds increased the synaptophysin-IR at doses previously established to be highly specific. Ipsapirone (10-9 M) was more effective on neuronal cell bodies staining and S100b (10 ng/ml) was more effective in increasing the number of synaptophysin-IR varicosities on neuronal processes. In addition both types of corticosteroid receptor agonists, at previously established specific doses, Ru28362 (10-8 M) and aldosterone (10-9 M) produced smaller increases compared to control groups in both the cell body staining and the number of varicosities. The effect of these differentiating factors on the expression of synaptophysin-IR suggests multiple regulation sites for producing and maintaining pre-synaptic elements in the brain.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
Issue number1
StatePublished - May 1996


  • Aldosterone
  • Differentiation
  • Ipsapirone
  • Ru28362
  • Synaptogenesis
  • Varicosity

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Enhanced synaptophysin immunoreactivity in rat hippocampal culture by 5-HT<sub>1A</sub> agonist, S100b, and corticosteroid receptor agonists'. Together they form a unique fingerprint.

Cite this