Enhancement of cancerous/normal tissue contrast via combined white light and fluorescence image processing: Initial investigation ex vivo

Angelos A. Kalitzeos; Azhar Zam; Florian Stelzle; Eckhart G. Hahn; Martin Raithel; Alexandre Douplik

doi:10.1117/12.831771

Enhancement of cancerous/normal tissue contrast via combined white light and fluorescence image processing: Initial investigation ex vivo

Angelos A. Kalitzeos, Azhar Zam, Florian Stelzle, Eckhart G. Hahn, Martin Raithel, Alexandre Douplik

General Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In the current study we try to optimise the cancerous/normal tissue contrast perception using white light and autofluorescence image processing. We deconvolved the RGB channels of both white light and autofluorescence images pixel by pixel and pointed out the best contrast enhancement technique, manipulating each channel seperately. By using false-color mapping according to a particular Look-Up Table, the borders between normal and cancerous area of the tissue are better delineated. Cancer tissue identification was confirmed by the histological examination that followed our investigation.

Original language	English (US)
Title of host publication	Novel Optical Instrumentation for Biomedical Applications IV
DOIs	https://doi.org/10.1117/12.831771
State	Published - 2009
Event	Novel Optical Instrumentation for Biomedical Applications IV - Munich, Germany Duration: Jun 14 2009 → Jun 17 2009

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7371
ISSN (Print)	1605-7422

Conference

Conference	Novel Optical Instrumentation for Biomedical Applications IV
Country/Territory	Germany
City	Munich
Period	6/14/09 → 6/17/09

Keywords

Autofluorescence
Clinical Applications
Endoscopic Imaging
Image Processing

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.831771

Cite this

Kalitzeos, A. A., Zam, A., Stelzle, F., Hahn, E. G., Raithel, M., & Douplik, A. (2009). Enhancement of cancerous/normal tissue contrast via combined white light and fluorescence image processing: Initial investigation ex vivo. In Novel Optical Instrumentation for Biomedical Applications IV Article 73710N (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7371). https://doi.org/10.1117/12.831771

Enhancement of cancerous/normal tissue contrast via combined white light and fluorescence image processing: Initial investigation ex vivo. / Kalitzeos, Angelos A.; Zam, Azhar; Stelzle, Florian et al.
Novel Optical Instrumentation for Biomedical Applications IV. 2009. 73710N (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7371).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kalitzeos, AA, Zam, A, Stelzle, F, Hahn, EG, Raithel, M & Douplik, A 2009, Enhancement of cancerous/normal tissue contrast via combined white light and fluorescence image processing: Initial investigation ex vivo. in Novel Optical Instrumentation for Biomedical Applications IV., 73710N, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7371, Novel Optical Instrumentation for Biomedical Applications IV, Munich, Germany, 6/14/09. https://doi.org/10.1117/12.831771

Kalitzeos AA, Zam A, Stelzle F, Hahn EG, Raithel M, Douplik A. Enhancement of cancerous/normal tissue contrast via combined white light and fluorescence image processing: Initial investigation ex vivo. In Novel Optical Instrumentation for Biomedical Applications IV. 2009. 73710N. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.831771

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abstract = "In the current study we try to optimise the cancerous/normal tissue contrast perception using white light and autofluorescence image processing. We deconvolved the RGB channels of both white light and autofluorescence images pixel by pixel and pointed out the best contrast enhancement technique, manipulating each channel seperately. By using false-color mapping according to a particular Look-Up Table, the borders between normal and cancerous area of the tissue are better delineated. Cancer tissue identification was confirmed by the histological examination that followed our investigation.",

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Enhancement of cancerous/normal tissue contrast via combined white light and fluorescence image processing: Initial investigation ex vivo

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

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