Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator

Chia Hung Chen; Aniruddh Sarkar; Yong Ak Song; Miles A. Miller; Sung Jae Kim; Linda G. Griffith; Douglas A. Lauffenburger; Jongyoon Han

doi:10.1021/ja2036628

Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator

Chia Hung Chen, Aniruddh Sarkar, Yong Ak Song, Miles A. Miller, Sung Jae Kim, Linda G. Griffith, Douglas A. Lauffenburger, Jongyoon Han

Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used.

Original language	English (US)
Pages (from-to)	10368-10371
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	133
Issue number	27
DOIs	https://doi.org/10.1021/ja2036628
State	Published - Jul 13 2011

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/ja2036628

Cite this

@article{3801d2f6760748f3933fa5aff0ebef2e,

title = "Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator",

abstract = "We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used.",

author = "Chen, {Chia Hung} and Aniruddh Sarkar and Song, {Yong Ak} and Miller, {Miles A.} and Kim, {Sung Jae} and Griffith, {Linda G.} and Lauffenburger, {Douglas A.} and Jongyoon Han",

year = "2011",

month = jul,

day = "13",

doi = "10.1021/ja2036628",

language = "English (US)",

volume = "133",

pages = "10368--10371",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "27",

}

TY - JOUR

T1 - Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator

AU - Chen, Chia Hung

AU - Sarkar, Aniruddh

AU - Song, Yong Ak

AU - Miller, Miles A.

AU - Kim, Sung Jae

AU - Griffith, Linda G.

AU - Lauffenburger, Douglas A.

AU - Han, Jongyoon

PY - 2011/7/13

Y1 - 2011/7/13

N2 - We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used.

AB - We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used.

UR - http://www.scopus.com/inward/record.url?scp=79960068110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960068110&partnerID=8YFLogxK

U2 - 10.1021/ja2036628

DO - 10.1021/ja2036628

M3 - Article

C2 - 21671557

AN - SCOPUS:79960068110

SN - 0002-7863

VL - 133

SP - 10368

EP - 10371

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 27

ER -

Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this