Enhancing/multiplexing protease assay with droplet based microfluidics using biomolecule concentrator

Chia Hung Chen, Aniruddh Sarkar, Yong Ak Song, Miles A. Miller, Sung Jae Kim, Linda G. Griffith, Douglas A. Lauffenburger, Jongyoon Han

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We introduce an integrated microfluidic device consisting of a biomolecule concentrator, picoinjector and a micro droplet generator. We used this platform to detect ultra-low levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used. Biomolecule concentrator enhances the limited sensitivity of low-abundance enzyme assays by concentrating sample solutions, while droplet generator confines concentrated reactants into a pico-liter volume for sustained high sensitivity enzyme reaction as well as enabling multiplexed assays with a limited sample volume. We demonstrated to screen five different protease sensors simultaneously to indicate specific protease MMP-9 with high sensitivity.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages2080-2082
Number of pages3
StatePublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Biomolecule concentrator
  • Emulsions
  • Multiplexed activity bioassay
  • Protease

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Chen, C. H., Sarkar, A., Song, Y. A., Miller, M. A., Kim, S. J., Griffith, L. G., Lauffenburger, D. A., & Han, J. (2011). Enhancing/multiplexing protease assay with droplet based microfluidics using biomolecule concentrator. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 2080-2082). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).