@inproceedings{47afeee99f3a409ea85d09740ae072e5,
title = "Enhancing/multiplexing protease assay with droplet based microfluidics using biomolecule concentrator",
abstract = "We introduce an integrated microfluidic device consisting of a biomolecule concentrator, picoinjector and a micro droplet generator. We used this platform to detect ultra-low levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used. Biomolecule concentrator enhances the limited sensitivity of low-abundance enzyme assays by concentrating sample solutions, while droplet generator confines concentrated reactants into a pico-liter volume for sustained high sensitivity enzyme reaction as well as enabling multiplexed assays with a limited sample volume. We demonstrated to screen five different protease sensors simultaneously to indicate specific protease MMP-9 with high sensitivity.",
keywords = "Biomolecule concentrator, Emulsions, Multiplexed activity bioassay, Protease",
author = "Chen, {Chia Hung} and Aniruddh Sarkar and Song, {Yong Ak} and Miller, {Miles A.} and Kim, {Sung Jae} and Griffith, {Linda G.} and Lauffenburger, {Douglas A.} and Jongyoon Han",
year = "2011",
language = "English (US)",
isbn = "9781618395955",
series = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",
pages = "2080--2082",
booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",
note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",
}