Abstract
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E. coli enterotoxin but requires neither radioactive reagents nor tissue culture techniques. The ELISA is easy to perform and is adaptable for use in small laboratories.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 439-444 |
| Number of pages | 6 |
| Journal | Journal of Clinical Microbiology |
| Volume | 6 |
| Issue number | 5 |
| State | Published - 1977 |
ASJC Scopus subject areas
- Microbiology (medical)