Equilibrium self-assembly of small RNA viruses

R. F. Bruinsma, M. Comas-Garcia, R. F. Garmann, A. Y. Grosberg

    Research output: Contribution to journalArticle

    Abstract

    We propose a description for the quasiequilibrium self-assembly of small, single-stranded (ss) RNA viruses whose capsid proteins (CPs) have flexible, positively charged, disordered tails that associate with the negatively charged RNA genome molecules. We describe the assembly of such viruses as the interplay between two coupled phase-transition-like events: the formation of the protein shell (the capsid) by CPs and the condensation of a large ss viral RNA molecule. Electrostatic repulsion between the CPs competes with attractive hydrophobic interactions and attractive interaction between neutralized RNA segments mediated by the tail groups. An assembly diagram is derived in terms of the strength of attractive interactions between CPs and between CPs and the RNA molecules. It is compared with the results of recent studies of viral assembly. We demonstrate that the conventional theory of self-assembly, which does describe the assembly of empty capsids, is in general not applicable to the self-assembly of RNA-encapsidating virions.

    Original languageEnglish (US)
    Article number032405
    JournalPhysical Review E
    Volume93
    Issue number3
    DOIs
    StatePublished - Mar 9 2016

    ASJC Scopus subject areas

    • Statistical and Nonlinear Physics
    • Statistics and Probability
    • Condensed Matter Physics

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  • Cite this

    Bruinsma, R. F., Comas-Garcia, M., Garmann, R. F., & Grosberg, A. Y. (2016). Equilibrium self-assembly of small RNA viruses. Physical Review E, 93(3), [032405]. https://doi.org/10.1103/PhysRevE.93.032405