Establishment of a leukemic lymphocyte culture from human aqueous humor

Nan Wang; Huanming Yang; Joel S. Schuman

doi:10.3109/02713689508999925

Establishment of a leukemic lymphocyte culture from human aqueous humor

Nan Wang, Huanming Yang, Joel S. Schuman

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

We have recently developed new techniques for culturing chronic lymphocytic leukemia (CLL) lymphocytes from human aqueous humor. We used leukemic lymphocytes collected from the aqueous humor of a patient with CLL and leukemic glaucoma. We grew these leukemic cells in combination with a feeder cell layer and other technical refinements. Microscopy and immunoassay indicate success in obtaining a homogenous population of B-type CLL cells through the 12th passage of the culture. No significant effect on cell growth was found with either of two mitogens (PWM and PHA), or between culture with and without autologous serum. Our new techniques for culturing leukemic cells derived from the aqueous humor provide a reliable resource for the study of chronic lymphocytic leukemia lymphocytes and leukemic glaucoma.

Original language	English (US)
Pages (from-to)	137-143
Number of pages	7
Journal	Current Eye Research
Volume	14
Issue number	2
DOIs	https://doi.org/10.3109/02713689508999925
State	Published - 1995

Keywords

Cell culture
Chronic lymphocytic leukemia
Feeder layer
Glaucoma
Human
Lymphocyte

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.3109/02713689508999925

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title = "Establishment of a leukemic lymphocyte culture from human aqueous humor",

abstract = "We have recently developed new techniques for culturing chronic lymphocytic leukemia (CLL) lymphocytes from human aqueous humor. We used leukemic lymphocytes collected from the aqueous humor of a patient with CLL and leukemic glaucoma. We grew these leukemic cells in combination with a feeder cell layer and other technical refinements. Microscopy and immunoassay indicate success in obtaining a homogenous population of B-type CLL cells through the 12th passage of the culture. No significant effect on cell growth was found with either of two mitogens (PWM and PHA), or between culture with and without autologous serum. Our new techniques for culturing leukemic cells derived from the aqueous humor provide a reliable resource for the study of chronic lymphocytic leukemia lymphocytes and leukemic glaucoma.",

keywords = "Cell culture, Chronic lymphocytic leukemia, Feeder layer, Glaucoma, Human, Lymphocyte",

author = "Nan Wang and Huanming Yang and Schuman, {Joel S.}",

note = "Funding Information: Supported in part by an unrestricted grant from Research to Prevent Blindness, Inc., and by the Glaucoma Foundation, NY.",

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AU - Wang, Nan

AU - Yang, Huanming

AU - Schuman, Joel S.

N1 - Funding Information: Supported in part by an unrestricted grant from Research to Prevent Blindness, Inc., and by the Glaucoma Foundation, NY.

PY - 1995

Y1 - 1995

N2 - We have recently developed new techniques for culturing chronic lymphocytic leukemia (CLL) lymphocytes from human aqueous humor. We used leukemic lymphocytes collected from the aqueous humor of a patient with CLL and leukemic glaucoma. We grew these leukemic cells in combination with a feeder cell layer and other technical refinements. Microscopy and immunoassay indicate success in obtaining a homogenous population of B-type CLL cells through the 12th passage of the culture. No significant effect on cell growth was found with either of two mitogens (PWM and PHA), or between culture with and without autologous serum. Our new techniques for culturing leukemic cells derived from the aqueous humor provide a reliable resource for the study of chronic lymphocytic leukemia lymphocytes and leukemic glaucoma.

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KW - Feeder layer

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Establishment of a leukemic lymphocyte culture from human aqueous humor

Abstract

Keywords

ASJC Scopus subject areas

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