Estimation of 3JHN-Hα and 3JHα-Hβ coupling constants from heteronuclear TOCSY spectra

Katiuscia Pagano, Federico Fogolari, Alessandra Corazza, Paolo Viglino, Gennaro Esposito

Research output: Contribution to journalArticlepeer-review


3J proton-proton coupling constants bear information on the intervening dihedral angles. Methods have been developed to derive this information from NMR spectra of proteins. Using series expansion of the time dependent density matrix, and exploiting the simple topology of amino acid spin-systems, formulae for estimation of 3JHN-Hα and 3 JHα-Hβ from HSQC-TOCSY spectra are derived. The results obtained on a protein entailing both α-helix and β-sheet secondary structure elements agree very well with J-coupling constants computed from the X-ray structure. The method compares well with existing methods and requires only 2D spectra which would be typically otherwise recorded for structural studies.

Original languageEnglish (US)
Pages (from-to)213-222
Number of pages10
JournalJournal of Biomolecular NMR
Issue number3
StatePublished - Nov 2007


  • Density matrix
  • Isotropic mixing
  • J-coupling
  • Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy


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