Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules

Saumya Saurabh, Suvrajit Maji, Marcel P. Bruchez

Research output: Contribution to journalArticlepeer-review


The ability to detect single molecules over the electronic noise requires high performance detector systems. Electron Multiplying Charge-Coupled Device (EMCCD) cameras have been employed successfully to image single molecules. Recently, scientific Complementary Metal Oxide Semiconductor (sCMOS) based cameras have been introduced with very low read noise at faster read out rates, smaller pixel sizes and a lower price compared to EMCCD cameras. In this study, we have compared the two technologies using two EMCCD and three sCMOS cameras to detect single Cy5 molecules. Our findings indicate that the sCMOS cameras perform similar to EMCCD cameras for detecting and localizing single Cy5 molecules.

Original languageEnglish (US)
Pages (from-to)7338-7349
Number of pages12
JournalOptics Express
Issue number7
StatePublished - Mar 26 2012

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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