Evidence for a novel voltage-activated channel in the outer mitochondrial membrane

K. W. Kinnally; H. Tedeschi; C. A. Mannella

doi:10.1016/0014-5793(87)80555-0

Evidence for a novel voltage-activated channel in the outer mitochondrial membrane

K. W. Kinnally, H. Tedeschi, C. A. Mannella

Research output: Contribution to journal › Article › peer-review

Abstract

Patch-clamp studies of the outer mitochondrial membrane indicate a voltage-dependent increase in conductance for potentials positive relative to the exterior of the mitochondrion. The time course of the conductance changes is consistent with an activation of channels. Voltage pulse experiments suggest that the activation phenomenon corresponds to assembly of the channels from subunits with disassembly occurring after recovery of the original conductance. Effects of temperature and concanavalin A on the voltage-induced conductances are also consistent with a channel assembly model.

Original language	English (US)
Pages (from-to)	83-87
Number of pages	5
Journal	FEBS Letters
Volume	226
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(87)80555-0
State	Published - Dec 21 1987

Keywords

Ion channel
Mitochondrial outer membrane
Patch clamp

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(87)80555-0

Cite this

@article{001c92b1909941898f45295fc207de7f,

title = "Evidence for a novel voltage-activated channel in the outer mitochondrial membrane",

abstract = "Patch-clamp studies of the outer mitochondrial membrane indicate a voltage-dependent increase in conductance for potentials positive relative to the exterior of the mitochondrion. The time course of the conductance changes is consistent with an activation of channels. Voltage pulse experiments suggest that the activation phenomenon corresponds to assembly of the channels from subunits with disassembly occurring after recovery of the original conductance. Effects of temperature and concanavalin A on the voltage-induced conductances are also consistent with a channel assembly model.",

keywords = "Ion channel, Mitochondrial outer membrane, Patch clamp",

author = "Kinnally, {K. W.} and H. Tedeschi and Mannella, {C. A.}",

note = "Funding Information: We are grateful to Drs David Carpenter and CharlesA llen for critical readingo f the manuscript and Robert Macey for discussionso f the data.T his study was supported by ONR grant N0001485-K-0681( to H.T.) and NSF grant DMB-8613702( to C.M.).",

year = "1987",

month = dec,

day = "21",

doi = "10.1016/0014-5793(87)80555-0",

language = "English (US)",

volume = "226",

pages = "83--87",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Evidence for a novel voltage-activated channel in the outer mitochondrial membrane

AU - Kinnally, K. W.

AU - Tedeschi, H.

AU - Mannella, C. A.

N1 - Funding Information: We are grateful to Drs David Carpenter and CharlesA llen for critical readingo f the manuscript and Robert Macey for discussionso f the data.T his study was supported by ONR grant N0001485-K-0681( to H.T.) and NSF grant DMB-8613702( to C.M.).

PY - 1987/12/21

Y1 - 1987/12/21

N2 - Patch-clamp studies of the outer mitochondrial membrane indicate a voltage-dependent increase in conductance for potentials positive relative to the exterior of the mitochondrion. The time course of the conductance changes is consistent with an activation of channels. Voltage pulse experiments suggest that the activation phenomenon corresponds to assembly of the channels from subunits with disassembly occurring after recovery of the original conductance. Effects of temperature and concanavalin A on the voltage-induced conductances are also consistent with a channel assembly model.

AB - Patch-clamp studies of the outer mitochondrial membrane indicate a voltage-dependent increase in conductance for potentials positive relative to the exterior of the mitochondrion. The time course of the conductance changes is consistent with an activation of channels. Voltage pulse experiments suggest that the activation phenomenon corresponds to assembly of the channels from subunits with disassembly occurring after recovery of the original conductance. Effects of temperature and concanavalin A on the voltage-induced conductances are also consistent with a channel assembly model.

KW - Ion channel

KW - Mitochondrial outer membrane

KW - Patch clamp

UR - http://www.scopus.com/inward/record.url?scp=0023662144&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023662144&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(87)80555-0

DO - 10.1016/0014-5793(87)80555-0

M3 - Article

C2 - 3691818

AN - SCOPUS:0023662144

SN - 0014-5793

VL - 226

SP - 83

EP - 87

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Evidence for a novel voltage-activated channel in the outer mitochondrial membrane

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this