Evidence that PAR-1 and PAR-2 mediate prostanoid-dependent contraction in isolated guinea-pig gallbladder

Michele Tognetto, Marcello Trevisani, Barbara Maggiore, Giuseppe Navarra, Alessandro Turini, Remo Guerrini, Nigel W. Bunnett, Pierangelo Geppetti, Selena Harrison

Research output: Contribution to journalArticle

Abstract

1 We have investigated the ability of protease-activated receptor-1 (PAR-1), PAR-2, PAR-3 and PAR-4 agonists to induce contractile responses in isolated guinea-pig gallbladder. Thrombin, trypsin, mouse PAR-1 activating (SFLLRN-NH2) peptide, and mouse PAR-2 activating (SLIGRL-NH2) and human PAR-2 activating (SLIGKV-NH2) peptides produced a concentration-dependent contractile response. 2 Mouse PAR-4 activating (GYPGKF-NH2 peptide, the mouse PAR-1 reverse (NRLLFS-NH2) peptide, the mouse PAR-2 reverse (LRGILS-NH2) and human PAR-2 reverse (VKGILS-NH2) peptides caused negligible contractile responses at the highest concentrations tested. 3 An additive effect was observed following the contractile response induced by either trypsin or thrombin, with the addition of a different PAR agonist (SFLLRN-NH2 and SLIGRL-NH2, respectively). Desensitization to PAR-2 activating peptide attenuated the response to trypsin but failed to attenuate the response to PAR-1 agonists, and conversely desensitization to PAR-1 attenuated the response to thrombin but failed to alter contractile responses to PAR-2 agonists. 4 The contractile responses produced by thrombin, trypsin, SFLLRN-NH2 and SLIGRL-NH2 were markedly reduced in the presence of the cyclo-oxygenase inhibitor, indomethacin, whilst the small contractile response produced by NRLLFS-NH2 and LRGILS-NH2 were insensitive to indomethacin. 5 The contractile responses to thrombin, trypsin, SFLLRN-NH2 and SLIGRL-NH2 were unaffected by the presence of: The non-selective muscarinic antagonist, atropine; the nitric oxide synthase inhibitor, L-NAME; the sodium channel blocker, tetrodotoxin; the combination of selective tachykinin NK1 and NK2 receptor antagonists, (S)-1-[2-[3-(3,4-dichlorphenyl)-1 (3-isopropoxyphenylacetyl) piperidin-3-yl] ethyl]-4-phenyl-1 azaniabicyclo [2.2.2] octane chloride (SR140333) and (S)-N-methyl-N-[4-acetylamino-4-phenylpiperidino-2-(3,4-dichlorophenyl)-butyl ] benzamide (SR48968), respectively. 6 The results indicate that PAR-1 and PAR-2 activation causes contractile responses in the guineapig gallbladder, an effect that is mediated principally by prostanoid release, and is independent of neural mechanisms.

Original languageEnglish (US)
Pages (from-to)689-694
Number of pages6
JournalBritish Journal of Pharmacology
Volume131
Issue number4
DOIs
StatePublished - Oct 1 2000

Keywords

  • Gallbladder
  • Guinea-pig
  • Prostanoids
  • Protease-activating receptor
  • Thrombin
  • Trypsin

ASJC Scopus subject areas

  • Pharmacology

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    Tognetto, M., Trevisani, M., Maggiore, B., Navarra, G., Turini, A., Guerrini, R., Bunnett, N. W., Geppetti, P., & Harrison, S. (2000). Evidence that PAR-1 and PAR-2 mediate prostanoid-dependent contraction in isolated guinea-pig gallbladder. British Journal of Pharmacology, 131(4), 689-694. https://doi.org/10.1038/sj.bjp.0703618