Evolving proteins of novel composition

Jin Kim Montclare; David A. Tirrell

doi:10.1002/anie.200600088

Evolving proteins of novel composition

Jin Kim Montclare, David A. Tirrell

Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

(Chemical Equation Presented) Changing its nature: Global incorporation of noncanonical amino acids can alter the behavior of proteins in useful ways. In some cases, however, replacement of natural amino acids by noncanonical analogues (blue bars in picture) can cause loss of protein stability. After several generations, functional proteins of non-natural composition were prepared through residue-specific incorporation combined with directed evolution.

Original language	English (US)
Pages (from-to)	4518-4521
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	45
Issue number	27
DOIs	https://doi.org/10.1002/anie.200600088
State	Published - Jul 3 2006

Keywords

Amino acids
Directed evolution
Enzymes
Protein engineering
Thermostability

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.200600088

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KW - Thermostability

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Evolving proteins of novel composition

Abstract

Keywords

ASJC Scopus subject areas

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