Evolving proteins of novel composition

Jin Kim Montclare; David A. Tirrell

doi:10.1002/anie.200600088

Evolving proteins of novel composition

Jin Kim Montclare, David A. Tirrell

Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

(Chemical Equation Presented) Changing its nature: Global incorporation of noncanonical amino acids can alter the behavior of proteins in useful ways. In some cases, however, replacement of natural amino acids by noncanonical analogues (blue bars in picture) can cause loss of protein stability. After several generations, functional proteins of non-natural composition were prepared through residue-specific incorporation combined with directed evolution.

Original language	English (US)
Pages (from-to)	4518-4521
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	45
Issue number	27
DOIs	https://doi.org/10.1002/anie.200600088
State	Published - Jul 3 2006

Keywords

Amino acids
Directed evolution
Enzymes
Protein engineering
Thermostability

ASJC Scopus subject areas

Catalysis
General Chemistry

Access to Document

10.1002/anie.200600088

Cite this

@article{48fcdf73bad24cbca3de25062415d07f,

title = "Evolving proteins of novel composition",

abstract = "(Chemical Equation Presented) Changing its nature: Global incorporation of noncanonical amino acids can alter the behavior of proteins in useful ways. In some cases, however, replacement of natural amino acids by noncanonical analogues (blue bars in picture) can cause loss of protein stability. After several generations, functional proteins of non-natural composition were prepared through residue-specific incorporation combined with directed evolution.",

keywords = "Amino acids, Directed evolution, Enzymes, Protein engineering, Thermostability",

author = "Montclare, {Jin Kim} and Tirrell, {David A.}",

year = "2006",

month = jul,

day = "3",

doi = "10.1002/anie.200600088",

language = "English (US)",

volume = "45",

pages = "4518--4521",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "27",

}

TY - JOUR

T1 - Evolving proteins of novel composition

AU - Montclare, Jin Kim

AU - Tirrell, David A.

PY - 2006/7/3

Y1 - 2006/7/3

N2 - (Chemical Equation Presented) Changing its nature: Global incorporation of noncanonical amino acids can alter the behavior of proteins in useful ways. In some cases, however, replacement of natural amino acids by noncanonical analogues (blue bars in picture) can cause loss of protein stability. After several generations, functional proteins of non-natural composition were prepared through residue-specific incorporation combined with directed evolution.

AB - (Chemical Equation Presented) Changing its nature: Global incorporation of noncanonical amino acids can alter the behavior of proteins in useful ways. In some cases, however, replacement of natural amino acids by noncanonical analogues (blue bars in picture) can cause loss of protein stability. After several generations, functional proteins of non-natural composition were prepared through residue-specific incorporation combined with directed evolution.

KW - Amino acids

KW - Directed evolution

KW - Enzymes

KW - Protein engineering

KW - Thermostability

UR - http://www.scopus.com/inward/record.url?scp=33746318719&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746318719&partnerID=8YFLogxK

U2 - 10.1002/anie.200600088

DO - 10.1002/anie.200600088

M3 - Article

C2 - 16763955

AN - SCOPUS:33746318719

SN - 1433-7851

VL - 45

SP - 4518

EP - 4521

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 27

ER -

Evolving proteins of novel composition

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this