Exopolysaccharide produced by potential probiotic Enterococcus faecium MS79: Characterization, bioactivities and rheological properties influenced by salt and pH

Mutamed Ayyash, Constantinos Stathopoulos, Basim Abu-Jdayil, Gennaro Esposito, Mohammad Baig, Mark S. Turner, Ahmad Salihin Baba, Vasso Apostolopoulos, Anas Al-Nabulsi, Tareq Osaili

Research output: Contribution to journalArticlepeer-review

Abstract

Microbial exopolysaccharide (EPS) is of interest due to their applications in food processing and potential health benefits following consumption. The objectives of this study were to: 1) isolate, purify and characterize EPS produced by Enterococcus faecium MS79 (EPS-MS79); 2) investigate bioactive properties of EPS-MS79; and 3) investigate the rheological properties of the EPS-MS79 as influenced by salt type and pH level. The average molecular weight of the EPS-MS79 was 8.3 × 105 Da and it is composed of the three monosaccharides arabinose, mannose, and glucose in a molar ratio of 0.8:1.7:11.3. Scavenging activities of EPS-MS79 were 76% and 85% as measured by DPPH and 26% and 44% as measured by ABTS. The antiproliferative activities against colon (72% and 77%) and breast (43% and 56%) cancer cell lines. EPS-MS79 inhibited the growth of bacterial pathogens by up to 3 logs compared to the control. All EPS-MS79 solutions displayed shear thinning behavior. The storage and loss moduli of all EPS-MS79 solutions increased alongside with the frequency increase. Salt type and pH level impacted on the rheological properties of EPS-MS79. The EPS-MS79 could be a potent prebiotic promoting health and rheological properties of foods.

Original languageEnglish (US)
Article number109741
JournalLWT - Food Science and Technology
Volume131
DOIs
StatePublished - Sep 2020

Keywords

  • Anticancer
  • Antidiabetic
  • Apparent viscosity
  • Probiotic
  • Thixotropic

ASJC Scopus subject areas

  • Food Science

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