Expression of heterologous genes in transgenic plants and plant cells using plant asparagine synthetase promoters

Gloria Coruzzi, Fong Ying Tsai

Research output: Contribution to journalArticle

Abstract

The identification and cloning of the gene(s) for plant asparagine synthetase Assigned to, an important enzyme involved in the formation of asparagine, a major nitrogen transport compound of higher plants is described. Expression vectors constructed with the AS coding sequence may be utilized to produce plant AS; to engineer herbicide resistant plants, salt/drought tolerant plants or pathogen resistant plants; as a dominant selectable marker; or to select for novel herbicides or compounds useful as agents that synchronize plant cells in culture. The promoter for plant AS, which directs high levels of gene expression and is induced in an organ specific manner and by darkness, is also described. The AS promoter may be used to direct the expression of heterologous coding sequences in appropriate hosts.

Original languageEnglish (US)
Number of pages1
JournalBiotechnology Advances
Volume15
Issue number3-4
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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