Expression of microRNA-30c and its target genes in human osteoblastic cells by nano-bioglass ceramic-treatment

A. Moorthi; S. Vimalraj; C. Avani; Zhiming He; Nicola C. Partridge; N. Selvamurugan

doi:10.1016/j.ijbiomac.2013.02.017

Expression of microRNA-30c and its target genes in human osteoblastic cells by nano-bioglass ceramic-treatment

A. Moorthi, S. Vimalraj, C. Avani, Zhiming He, Nicola C. Partridge, N. Selvamurugan

Molecular Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

Osteoblast differentiation is tightly regulated by post transcriptional regulators such as microRNAs (miRNAs). Several bioactive materials including nano-bioglass ceramic particles (nBGC) influence differentiation of the osteoblasts, but the molecular mechanisms of nBGC-stimulation of osteoblast differentiation via miRNAs are not yet determined. In this study, we identified that nBGC-treatment stimulated miR-30c expression in human osteoblastic cells (MG63). The bioinformatics tools identified its regulatory network, molecular function, biological processes and its target genes involved in negative regulation of osteoblast differentiation. TGIF2 and HDAC4 were found to be its putative target genes and their expression was down regulated by nBGC-treatment in MG63 cells. Thus, this study advances our understanding of nBGC action on bone cells and supports utilization of nBGC in bone tissue engineering.

Original language	English (US)
Pages (from-to)	181-185
Number of pages	5
Journal	International Journal of Biological Macromolecules
Volume	56
DOIs	https://doi.org/10.1016/j.ijbiomac.2013.02.017
State	Published - May 2013

Keywords

Bioglass ceramic
HDAC4
MiR-30c
Osteoblast
Runx2

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology
Economics and Econometrics
General Energy

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10.1016/j.ijbiomac.2013.02.017

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title = "Expression of microRNA-30c and its target genes in human osteoblastic cells by nano-bioglass ceramic-treatment",

abstract = "Osteoblast differentiation is tightly regulated by post transcriptional regulators such as microRNAs (miRNAs). Several bioactive materials including nano-bioglass ceramic particles (nBGC) influence differentiation of the osteoblasts, but the molecular mechanisms of nBGC-stimulation of osteoblast differentiation via miRNAs are not yet determined. In this study, we identified that nBGC-treatment stimulated miR-30c expression in human osteoblastic cells (MG63). The bioinformatics tools identified its regulatory network, molecular function, biological processes and its target genes involved in negative regulation of osteoblast differentiation. TGIF2 and HDAC4 were found to be its putative target genes and their expression was down regulated by nBGC-treatment in MG63 cells. Thus, this study advances our understanding of nBGC action on bone cells and supports utilization of nBGC in bone tissue engineering.",

keywords = "Bioglass ceramic, HDAC4, MiR-30c, Osteoblast, Runx2",

author = "A. Moorthi and S. Vimalraj and C. Avani and Zhiming He and Partridge, {Nicola C.} and N. Selvamurugan",

note = "Funding Information: This work was supported by the grants from China Agriculture Research System-Green Manure ( CARS-22 ) and the National Science Foundation of China ( 31771964 & 31571791 ).",

year = "2013",

month = may,

doi = "10.1016/j.ijbiomac.2013.02.017",

language = "English (US)",

volume = "56",

pages = "181--185",

journal = "International Journal of Biological Macromolecules",

issn = "0141-8130",

publisher = "Elsevier B.V.",

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T1 - Expression of microRNA-30c and its target genes in human osteoblastic cells by nano-bioglass ceramic-treatment

AU - Moorthi, A.

AU - Vimalraj, S.

AU - Avani, C.

AU - He, Zhiming

AU - Partridge, Nicola C.

AU - Selvamurugan, N.

N1 - Funding Information: This work was supported by the grants from China Agriculture Research System-Green Manure ( CARS-22 ) and the National Science Foundation of China ( 31771964 & 31571791 ).

PY - 2013/5

Y1 - 2013/5

N2 - Osteoblast differentiation is tightly regulated by post transcriptional regulators such as microRNAs (miRNAs). Several bioactive materials including nano-bioglass ceramic particles (nBGC) influence differentiation of the osteoblasts, but the molecular mechanisms of nBGC-stimulation of osteoblast differentiation via miRNAs are not yet determined. In this study, we identified that nBGC-treatment stimulated miR-30c expression in human osteoblastic cells (MG63). The bioinformatics tools identified its regulatory network, molecular function, biological processes and its target genes involved in negative regulation of osteoblast differentiation. TGIF2 and HDAC4 were found to be its putative target genes and their expression was down regulated by nBGC-treatment in MG63 cells. Thus, this study advances our understanding of nBGC action on bone cells and supports utilization of nBGC in bone tissue engineering.

AB - Osteoblast differentiation is tightly regulated by post transcriptional regulators such as microRNAs (miRNAs). Several bioactive materials including nano-bioglass ceramic particles (nBGC) influence differentiation of the osteoblasts, but the molecular mechanisms of nBGC-stimulation of osteoblast differentiation via miRNAs are not yet determined. In this study, we identified that nBGC-treatment stimulated miR-30c expression in human osteoblastic cells (MG63). The bioinformatics tools identified its regulatory network, molecular function, biological processes and its target genes involved in negative regulation of osteoblast differentiation. TGIF2 and HDAC4 were found to be its putative target genes and their expression was down regulated by nBGC-treatment in MG63 cells. Thus, this study advances our understanding of nBGC action on bone cells and supports utilization of nBGC in bone tissue engineering.

KW - Bioglass ceramic

KW - HDAC4

KW - MiR-30c

KW - Osteoblast

KW - Runx2

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AN - SCOPUS:84875121424

SN - 0141-8130

VL - 56

SP - 181

EP - 185

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Expression of microRNA-30c and its target genes in human osteoblastic cells by nano-bioglass ceramic-treatment

Abstract

Keywords

ASJC Scopus subject areas

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