Expression of microRNA-30c and its target genes in human osteoblastic cells by nano-bioglass ceramic-treatment

A. Moorthi, S. Vimalraj, C. Avani, Zhiming He, Nicola C. Partridge, N. Selvamurugan

Research output: Contribution to journalArticlepeer-review

Abstract

Osteoblast differentiation is tightly regulated by post transcriptional regulators such as microRNAs (miRNAs). Several bioactive materials including nano-bioglass ceramic particles (nBGC) influence differentiation of the osteoblasts, but the molecular mechanisms of nBGC-stimulation of osteoblast differentiation via miRNAs are not yet determined. In this study, we identified that nBGC-treatment stimulated miR-30c expression in human osteoblastic cells (MG63). The bioinformatics tools identified its regulatory network, molecular function, biological processes and its target genes involved in negative regulation of osteoblast differentiation. TGIF2 and HDAC4 were found to be its putative target genes and their expression was down regulated by nBGC-treatment in MG63 cells. Thus, this study advances our understanding of nBGC action on bone cells and supports utilization of nBGC in bone tissue engineering.

Original languageEnglish (US)
Pages (from-to)181-185
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume56
DOIs
StatePublished - May 2013

Keywords

  • Bioglass ceramic
  • HDAC4
  • MiR-30c
  • Osteoblast
  • Runx2

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

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