TY - JOUR
T1 - Expression of serotonin receptors in allergic contact eczematous human skin
AU - Lundeberg, Lena
AU - El-Nour, Husameldin
AU - Mohabbati, Sheila
AU - Morales, Marisela
AU - Azmitia, Efraim
AU - Nordlind, Klas
N1 - Funding Information:
Acknowledgements This study was supported by grants from the Swedish Foundation for Health Care Sciences and Allergy Re- search, the Swedish Society of Medicine, the Ekhaga Foundation, MH55250 (E.A.), the Magnus Bergwall Foundation, the Welander/ Finsen Foundation, and the Karolinska Institute. The technical skill of Mrs. Eva Hagforsen is gratefully acknowledged.
PY - 2002
Y1 - 2002
N2 - The expression of the serotonin (5-HT) receptors 5-HT1AR, 5-HT2AR and 5-HT3R was investigated in allergic contact eczematous human skin by an indirect fluorescence method. 5-HT1AR expression was found in basal epidermal NK1-beteb-positive cells, which were more elongated and showed longer dendritic processes in contact eczematous skin than in control skin. Immunoreactivity for 5-HT1AR was also found in the upper part of the epidermis, with no difference between eczematous and control skin. 5-HT1AR expression was also found in 33.3±6.5% and 63.7±11.3% of papillary dermal mononuclear cells in inflamed skin and control skin, respectively (P<0.001), as well as in vessel walls. Some of these mononuclear cells were tryptase-positive, and found in both eczematous and control skin. 5-HT2AR-positive cells were found in the upper part of the epidermis in eczematous skin, but were more evenly distributed in the epidermis of control skin. In addition, inflammatory dermal mononuclear cells and vessel walls showed immunoreactivity for this receptor. 5-HT3R expression was found in the basal epidermal layer of eczematous and control skin. These findings indicate a plasticity in the effects of serotonin, especially regarding 5-HT1AR, in allergic contact eczematous skin.
AB - The expression of the serotonin (5-HT) receptors 5-HT1AR, 5-HT2AR and 5-HT3R was investigated in allergic contact eczematous human skin by an indirect fluorescence method. 5-HT1AR expression was found in basal epidermal NK1-beteb-positive cells, which were more elongated and showed longer dendritic processes in contact eczematous skin than in control skin. Immunoreactivity for 5-HT1AR was also found in the upper part of the epidermis, with no difference between eczematous and control skin. 5-HT1AR expression was also found in 33.3±6.5% and 63.7±11.3% of papillary dermal mononuclear cells in inflamed skin and control skin, respectively (P<0.001), as well as in vessel walls. Some of these mononuclear cells were tryptase-positive, and found in both eczematous and control skin. 5-HT2AR-positive cells were found in the upper part of the epidermis in eczematous skin, but were more evenly distributed in the epidermis of control skin. In addition, inflammatory dermal mononuclear cells and vessel walls showed immunoreactivity for this receptor. 5-HT3R expression was found in the basal epidermal layer of eczematous and control skin. These findings indicate a plasticity in the effects of serotonin, especially regarding 5-HT1AR, in allergic contact eczematous skin.
KW - Contact eczema
KW - Receptors
KW - Serotonin
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U2 - 10.1007/s00403-002-0350-y
DO - 10.1007/s00403-002-0350-y
M3 - Article
C2 - 12522576
AN - SCOPUS:0036454054
SN - 0340-3696
VL - 294
SP - 393
EP - 398
JO - Archives of Dermatological Research
JF - Archives of Dermatological Research
IS - 9
ER -