TY - JOUR
T1 - Extracellular matrix
T2 - an immunological and biochemical (CAT and TOH activity) survey of in vitro differentiation of isolated amphibian neuroblasts
AU - Huang, S.
AU - Saint-Jeannet, J. P.
AU - Kan, P.
AU - Duprat, A. M.
N1 - Funding Information:
We are indebted to Dr. J.P. Thiery and Dr. J.C. Boucaut who kindly provided antibodies against FN and FN-R, to Dr. P. Cochard who made a critical reading of the manuscript. The expert technical assistance of F. Foulquier and photographic assistance of C. Daguzan were gratefully appreciated. We want to thank Simon Jarman for reviewing the English manuscript. This work was supported by grants from the CNRS, the MRES and the EEC.
PY - 1990/6
Y1 - 1990/6
N2 - After neural induction certain cells in the neuroepithelium immediately acquire the property to express certain neural phenotypes (Duprat et al., 1984, 1987). However, the activity of almost all the specific enzymes involved in the biosynthesis of neurotransmitters is considerably higher when neurectodermal cells are cultured with chordamesodermal cells than when they are cultured alone. The stimulating effects of chordamesoderm do not appear to be due to diffusible factors (Duprat et al., 1985b). The present study was designed to investigate the role of extracellular matrix components in neuronal cell differentiation. We showed that the extracellular matrix cannot replace chordamesoderm in stimulating the biochemical differentiation of neuroblasts, although fibronectin and especially laminin stimulate morphological differentiation. We suggest that interaction between neuronal and non-neuronal cells plays an important part in functional biochemical differentiation, whereas the molecules of extracellular matrix are important for morphological differentiation.
AB - After neural induction certain cells in the neuroepithelium immediately acquire the property to express certain neural phenotypes (Duprat et al., 1984, 1987). However, the activity of almost all the specific enzymes involved in the biosynthesis of neurotransmitters is considerably higher when neurectodermal cells are cultured with chordamesodermal cells than when they are cultured alone. The stimulating effects of chordamesoderm do not appear to be due to diffusible factors (Duprat et al., 1985b). The present study was designed to investigate the role of extracellular matrix components in neuronal cell differentiation. We showed that the extracellular matrix cannot replace chordamesoderm in stimulating the biochemical differentiation of neuroblasts, although fibronectin and especially laminin stimulate morphological differentiation. We suggest that interaction between neuronal and non-neuronal cells plays an important part in functional biochemical differentiation, whereas the molecules of extracellular matrix are important for morphological differentiation.
KW - Amphibian
KW - Choline acetyltransferase
KW - Extracellular matrix
KW - Neural differentiation
KW - Neurogenesis
KW - Tyrosine hydroxylase
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U2 - 10.1016/0922-3371(90)90141-I
DO - 10.1016/0922-3371(90)90141-I
M3 - Article
C2 - 1976426
AN - SCOPUS:0025317015
SN - 0922-3371
VL - 30
SP - 219
EP - 233
JO - Cell Differentiation and Development
JF - Cell Differentiation and Development
IS - 3
ER -