@article{ef0c8bd2813b4063b1770df4581e81d9,
title = "Extracellular matrix couples the convergence movements of mesoderm and neural plate during the early stages of neurulation",
abstract = "Background: During the initial stages zebrafish neurulation, neural plate cells undergo highly coordinated movements before they assemble into a multicellular solid neural rod. We have previously identified that the underlying mesoderm is critical to ensure such coordination and generate correct neural tube organization. However, how intertissue coordination is achieved in vivo during zebrafish neural tube morphogenesis is unknown. Results: In this work, we use quantitative live imaging to study the coordinated movements of neural ectoderm and mesoderm during dorsal tissue convergence. We show the extracellular matrix components laminin and fibronectin that lie between mesoderm and neural plate act to couple the movements of neural plate and mesoderm during early stages of neurulation and to maintain the close apposition of these two tissues. Conclusions: Our study highlights the importance of the extracellular matrix proteins laminin and libronectin in coupling the movements and spatial proximity of mesoderm and neuroectoderm during the morphogenetic movements of neurulation. Developmental Dynamics 245:580-589, 2016.",
keywords = "Extracellular matrix, Morphogenesis, Neurulation, Zebrafish",
author = "Claudio Araya and Carlos Carmona-Fontaine and Clarke, {Jonathan D.W.}",
note = "Funding Information: We thank all present and past members of the Clarke and Araya labs for critical reading and helpful discussion. We thank Marta Costa for the images in Figure 5A and B. We also thank the KCL Fish Facility staff for help with husbandry. This work was supported by grants from the BBSRC to J.D.W.C. and Fondecyt, Centro Interdisciplinario de Estudios del Sistema Nervioso (CISNe, UACh), and ECOS/Conicyt to C.A. The authors declare that they have no competing interests. C.A. performed and analyzed the majority of the experiments and design experimental strategy. C.C.F. helped to analyze the data of Figures 5 and 6. J.D.W.C. oversaw the whole project and wrote the manuscript together with C.A. All authors read and approved the final manuscript. Funding Information: We thank all present and past members of the Clarke and Araya labs for critical reading and helpful discussion. We thank Marta Costa for the images in Figure 5A and B. We also thank the KCL Fish Facility staff for help with husbandry. This work was supported by grants from the BBSRC to J.D.W.C. and Fondecyt, Centro Interdisciplinario de Estudios del Sistema Nervioso (CISNe, UACh), and ECOS/Conicyt to C.A. The authors declare that they have no competing interests. C.A. performed and analyzed the majority of the experiments and design experimental strategy. C.C.F. helped to analyze the data of Figures 5 and 6. J.D.W.C. oversaw the whole project and wrote the manuscript together with C.A. All authors read and approved the final manuscript. Publisher Copyright: {\textcopyright} 2016 Wiley Periodicals, Inc.",
year = "2016",
month = may,
day = "1",
doi = "10.1002/dvdy.24401",
language = "English (US)",
volume = "245",
pages = "580--589",
journal = "American Journal of Anatomy",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "5",
}