@article{a0642c20c41242db9afb53b27be492f1,
title = "Extrinsic activin signaling cooperates with an intrinsic temporal program to increase mushroom body neuronal diversity",
abstract = "Temporal patterning of neural progenitors leads to the sequential production of diverse neurons. To understand how extrinsic cues influence intrinsic temporal programs, we studied Drosophila mushroom body progenitors (neuroblasts) that sequentially produce only three neuronal types: γ, then α'β', followed by αβ. Opposing gradients of two RNA-binding proteins Imp and Syp comprise the intrinsic temporal program. Extrinsic activin signaling regulates the production of α'β' neurons but whether it affects the intrinsic temporal program was not known. We show that the activin ligand Myoglianin from glia regulates the temporal factor Imp in mushroom body neuroblasts. Neuroblasts missing the activin receptor Baboon have a delayed intrinsic program as Imp is higher than normal during the α'β' temporal window, causing the loss of α'β' neurons, a decrease in αβ neurons, and a likely increase in γ neurons, without affecting the overall number of neurons produced. Our results illustrate that an extrinsic cue modifies an intrinsic temporal program to increase neuronal diversity. ",
keywords = "Activins/metabolism, Animals, Drosophila Proteins/genetics, Drosophila melanogaster/genetics, Ligands, Mushroom Bodies/physiology, Neural Stem Cells/physiology, Neurons/physiology, RNA-Binding Proteins/genetics, Signal Transduction, Transforming Growth Factor beta/genetics",
author = "Rossi, {Anthony M.} and Claude Desplan",
note = "Funding Information: We would like to thank the fly community, the Bloomington and the DGRC stock centers for flies and reagents; Nikos Konstantinides for discussion throughout the project and comments on the manuscript, and the three reviewers for constructive feedback on our paper; Tzumin Lee for sharing data prior to publication and for discussions; Tzumin Lee, Lynn Riddiford, Oren Schuldine, Cedric Maurange and Michael B O{\textquoteright}Connor for fly stocks and antibodies; And all the Desplan lab members for their discussion and comments on the manuscript. This work was supported by grants from NIH (R01 EY017916 and R21 NS095288) and from NYSTEM (DOH01-C32604GG) to CD. AMR was partly supported by funding from NIH (T32 HD007520), and by NYU{\textquoteright}s GSAS MacCracken Program and a Dean{\textquoteright}s Dissertation Fellowship. National Eye Institute R01 EY017916 Claude Desplan National Institute of Neurological Disorders and Stroke R21 NS095288 Claude Desplan National Institutes of Health T32 HD007520 Anthony M Rossi New York University GSAS MacCracken Program Anthony M Rossi New York State Stem Cell Science DOH01-C32604GG Claude Desplan. Funding Information: We would like to thank the fly community, the Bloomington and the DGRC stock centers for flies and reagents; Nikos Konstantinides for discussion throughout the project and comments on the manuscript, and the three reviewers for constructive feedback on our paper; Tzumin Lee for sharing data prior to publication and for discussions; Tzumin Lee, Lynn Riddiford, Oren Schuldine, Cedric Maurange and Michael B O{\textquoteright}Connor for fly stocks and antibodies; And all the Desplan lab members for their discussion and comments on the manuscript. Funding: This work was supported by grants from NIH (R01 EY017916 and R21 NS095288) and from NYSTEM (DOH01-C32604GG) to CD. AMR was partly supported by funding from NIH (T32 HD007520), and by NYU{\textquoteright}s GSAS MacCracken Program and a Dean{\textquoteright}s Dissertation Fellowship. Publisher Copyright: {\textcopyright} Rossi and Desplan.",
year = "2020",
month = jul,
doi = "10.7554/eLife.58880",
language = "English (US)",
volume = "9",
pages = "1--23",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}