Fabrication of an oriented lectin microarray

Daniel C. Propheter; Ku Lung Hsu; Lara K. Mahal

doi:10.1002/cbic.201000106

Fabrication of an oriented lectin microarray

Daniel C. Propheter, Ku Lung Hsu, Lara K. Mahal

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

To tag or not to tag: An oriented lectin microarray utilizing recombinant lectins with the standard prokaryotic expression tag glutathione S-transferase has been reported. The oriented lectin microarray improves the overall sensitivity and limits of detection by site-specific orientation of recombinant lectins. This continual improvement of the lectin microarray technology enables a deeper understanding of the biological function of glycans. (Figure Presented)

Original language	English (US)
Pages (from-to)	1203-1207+1157
Journal	ChemBioChem
Volume	11
Issue number	9
DOIs	https://doi.org/10.1002/cbic.201000106
State	Published - Jun 14 2010

Keywords

Carbohydrates
GST-tagged proteins
Glycomics
Microarrays
Recombinant lectin

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.201000106

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AB - To tag or not to tag: An oriented lectin microarray utilizing recombinant lectins with the standard prokaryotic expression tag glutathione S-transferase has been reported. The oriented lectin microarray improves the overall sensitivity and limits of detection by site-specific orientation of recombinant lectins. This continual improvement of the lectin microarray technology enables a deeper understanding of the biological function of glycans. (Figure Presented)

KW - Carbohydrates

KW - GST-tagged proteins

KW - Glycomics

KW - Microarrays

KW - Recombinant lectin

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Fabrication of an oriented lectin microarray

Abstract

Keywords

ASJC Scopus subject areas

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