Fabrication of gelatin nanopatterns for cell culture studies

S. S. Li, J. Shi, L. Liu, J. J. Li, L. M. Jiang, C. X. Luo, K. Kamei, Y. Chen

Research output: Contribution to journalArticlepeer-review


We developed a gel-embossing technique which could be used to fabricate gelatin nanopatterns for cell culture studies. A gel of gelatin solution was dropped on the surface of a glass slide and embossed using a gas permeable stamp made of polydimethylsiloxane (PDMS). After drying in a desiccator, nanostructure patterns could be replicated from the stamp into the gelatin layer. After crosslinking in a 10% formaldehyde solution in phosphate buffered saline (PBS), the embossed gelatin layer could be used for cell culture studies. The gel-embossed patterns before and after crosslinking were characterized by atomic force microscopy, revealing significant changes of the pattern morphology introduced by both gel-emboss and crosslink processes. Nonetheless, strong effects of cell elongation and fast cell growth were observed for the proof-of-concept using NIH-3T3 cells cultured on gelatin nanogrooves.

Original languageEnglish (US)
Pages (from-to)70-74
Number of pages5
JournalMicroelectronic Engineering
StatePublished - 2013


  • Cell culture
  • Gel-embossing
  • Gelatin
  • Nanopatterns

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Electrical and Electronic Engineering


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