Fabrication of peg-well microarrays on a track-etched membrane forsingle cell selection

Maryam Mirzaei, Mateu Pla-Roca, Mohammad A. Qasaimeh, Setareh Ghorbanian, Roozbeh Safavieh, Satya Prakash, David Juncker

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Isolation and expansion of a single productive cell from a large population of non-productive cells is widely used in biotechnology for many different purposes i.e. in monoclonal antibody (Ab) production. Conventional methods need the formation of large colonies, which require weeks to grow. Here, we propose a new microfluidic system for the identification of productive clones using a few cells only, that can be obtained within a few days. A polyethylene glycol (PEG) microwell array with a track-etched membrane as the bottom is used atop a PDMS substrate coated with a capture Ab. Proteins released by cells diffuse through the track-etched membrane, bind to the surface beneath the cell, and may subsequently be localized by incubating the PDMS substrate with a fluorescently tagged detection Ab; the localization of a bright spot corresponds to a productive cell line which can then be retrieved.

Original languageEnglish (US)
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1168-1170
Number of pages3
ISBN (Print)9780979806421
StatePublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Keywords

  • PEG-well microarrays
  • Single cell selection
  • Track-etched membrane

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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