TY - GEN
T1 - Fabrication of peg-well microarrays on a track-etched membrane forsingle cell selection
AU - Mirzaei, Maryam
AU - Pla-Roca, Mateu
AU - Qasaimeh, Mohammad A.
AU - Ghorbanian, Setareh
AU - Safavieh, Roozbeh
AU - Prakash, Satya
AU - Juncker, David
PY - 2009
Y1 - 2009
N2 - Isolation and expansion of a single productive cell from a large population of non-productive cells is widely used in biotechnology for many different purposes i.e. in monoclonal antibody (Ab) production. Conventional methods need the formation of large colonies, which require weeks to grow. Here, we propose a new microfluidic system for the identification of productive clones using a few cells only, that can be obtained within a few days. A polyethylene glycol (PEG) microwell array with a track-etched membrane as the bottom is used atop a PDMS substrate coated with a capture Ab. Proteins released by cells diffuse through the track-etched membrane, bind to the surface beneath the cell, and may subsequently be localized by incubating the PDMS substrate with a fluorescently tagged detection Ab; the localization of a bright spot corresponds to a productive cell line which can then be retrieved.
AB - Isolation and expansion of a single productive cell from a large population of non-productive cells is widely used in biotechnology for many different purposes i.e. in monoclonal antibody (Ab) production. Conventional methods need the formation of large colonies, which require weeks to grow. Here, we propose a new microfluidic system for the identification of productive clones using a few cells only, that can be obtained within a few days. A polyethylene glycol (PEG) microwell array with a track-etched membrane as the bottom is used atop a PDMS substrate coated with a capture Ab. Proteins released by cells diffuse through the track-etched membrane, bind to the surface beneath the cell, and may subsequently be localized by incubating the PDMS substrate with a fluorescently tagged detection Ab; the localization of a bright spot corresponds to a productive cell line which can then be retrieved.
KW - PEG-well microarrays
KW - Single cell selection
KW - Track-etched membrane
UR - http://www.scopus.com/inward/record.url?scp=84901791505&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901791505&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901791505
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1168
EP - 1170
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -