Faster voltage-dependent activation of Na+ channels in growth cones versus somata of neuroblastoma N1E-115 cells

Jing Zhang; Leslie M. Loew; Robert M. Davidson

doi:10.1016/S0006-3495(96)79443-2

Faster voltage-dependent activation of Na⁺ channels in growth cones versus somata of neuroblastoma N1E-115 cells

Jing Zhang, Leslie M. Loew, Robert M. Davidson

Periodontology and Implant Dentistry

Research output: Contribution to journal › Article › peer-review

Abstract

Kinetics of voltage-gated ionic channels fundamentally reflect the response of the channels to local electric fields. In this report cell- attached patch-clamp studies reveal that the voltage-dependent activation rate of sodium channels residing in the growth cone membrane differs from that of soma sodium channels in differentiating N1E-115 neuroblastoma cells. Because other electrophysiological properties of these channels do not differ, this finding may be a reflection of the difference in intramembrane electric field in these two regions of the cell. This represents a new mechanism for channels to attain a range of activities both within and between cells.

Original language	English (US)
Pages (from-to)	2501-2508
Number of pages	8
Journal	Biophysical journal
Volume	71
Issue number	5
DOIs	https://doi.org/10.1016/S0006-3495(96)79443-2
State	Published - Nov 1996

ASJC Scopus subject areas

Biophysics

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10.1016/S0006-3495(96)79443-2

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title = "Faster voltage-dependent activation of Na+ channels in growth cones versus somata of neuroblastoma N1E-115 cells",

abstract = "Kinetics of voltage-gated ionic channels fundamentally reflect the response of the channels to local electric fields. In this report cell- attached patch-clamp studies reveal that the voltage-dependent activation rate of sodium channels residing in the growth cone membrane differs from that of soma sodium channels in differentiating N1E-115 neuroblastoma cells. Because other electrophysiological properties of these channels do not differ, this finding may be a reflection of the difference in intramembrane electric field in these two regions of the cell. This represents a new mechanism for channels to attain a range of activities both within and between cells.",

author = "Jing Zhang and Loew, {Leslie M.} and Davidson, {Robert M.}",

note = "Funding Information: We thank Dr. Mei-de Wei for providing us with NlE-1 15 neuroblastoma cells and Drs. Hermes Yeh, Mary Ann Epstein, Barbara Ehrlich, and Lawrence B. Cohen for helpful comments. This study was supported by grants GM 35063 and ES05973 (to LML) and DE09662 (to RMD) from the National Institutes of Health.",

year = "1996",

month = nov,

doi = "10.1016/S0006-3495(96)79443-2",

language = "English (US)",

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journal = "Biophysical Journal",

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T1 - Faster voltage-dependent activation of Na+ channels in growth cones versus somata of neuroblastoma N1E-115 cells

AU - Zhang, Jing

AU - Loew, Leslie M.

AU - Davidson, Robert M.

N1 - Funding Information: We thank Dr. Mei-de Wei for providing us with NlE-1 15 neuroblastoma cells and Drs. Hermes Yeh, Mary Ann Epstein, Barbara Ehrlich, and Lawrence B. Cohen for helpful comments. This study was supported by grants GM 35063 and ES05973 (to LML) and DE09662 (to RMD) from the National Institutes of Health.

PY - 1996/11

Y1 - 1996/11

N2 - Kinetics of voltage-gated ionic channels fundamentally reflect the response of the channels to local electric fields. In this report cell- attached patch-clamp studies reveal that the voltage-dependent activation rate of sodium channels residing in the growth cone membrane differs from that of soma sodium channels in differentiating N1E-115 neuroblastoma cells. Because other electrophysiological properties of these channels do not differ, this finding may be a reflection of the difference in intramembrane electric field in these two regions of the cell. This represents a new mechanism for channels to attain a range of activities both within and between cells.

AB - Kinetics of voltage-gated ionic channels fundamentally reflect the response of the channels to local electric fields. In this report cell- attached patch-clamp studies reveal that the voltage-dependent activation rate of sodium channels residing in the growth cone membrane differs from that of soma sodium channels in differentiating N1E-115 neuroblastoma cells. Because other electrophysiological properties of these channels do not differ, this finding may be a reflection of the difference in intramembrane electric field in these two regions of the cell. This represents a new mechanism for channels to attain a range of activities both within and between cells.

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Faster voltage-dependent activation of Na⁺ channels in growth cones versus somata of neuroblastoma N1E-115 cells

Abstract

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