TY - JOUR
T1 - Fetal cleft lip repair in rabbits
T2 - Histology and role of hyaluronic acid
AU - Stern, Michael
AU - Schmidt, Brian
AU - Dodson, Thomas B.
AU - Stern, Robert
AU - Kaban, Leonard B.
N1 - Funding Information:
Received from the University of California, San Francisco. * Resident, Department of Oral and Maxillofacial Surgery/PhD in Oral Biology Program. $ Third-Year Dental Student. $ Adjunct Assistant Clinical Professor, Department of Oral and Maxillofacial Surgery. 0 Professor of Pathology, Department of Pathology. ‘1P rofessor and Chair, Department of Oral and Maxillofacial Surgery. Supported by an American Association of Oral and Maxillofacial Surgeons Research support grant (LBK), NIH R03 DE 93250-l (LBK) NIH HD 25505 (RS), and NIDR T32 DE07204 (MS). Presented at the 72nd annual meeting of the American Association of Oral and Maxillofacial Surgeons, New Orleans, LA, September 12-16, 1990. Address and reprint requests to Dr Kaban: Department of Oral and Maxillofacial Surgery, S-738 University of California, San Francisco, San Francisco, CA 94 143-0440.
PY - 1992/3
Y1 - 1992/3
N2 - This study examines the histologic and biochemical features of wound healing in a cleft lip model in the mid-third-trimester fetal rabbit. At days 1, 2, and 4 after the procedure, control, unrepaired, and repaired fetal heads were obtained, sectioned, and stained for histologic examination. The localization of hyaluronic acid in the wound was documented using a cartilage-derived hyaluronic acid-binding protein. In both repaired and unrepaired wounds, the fetal cleft healed without inflammatory cell infiltration or scar formation. Six months after birth, the repaired cleft showed complete regeneration of muscle across the wound and the collagen fibers were of normal density and orientation. Decreased hyaluronic acid deposition was observed in unrepaired clefts as compared with adjacent tissue; no such difference was detected in repaired clefts. Our findings support the hypothesis that a cleft lip repaired in utero heals without the scarring that accompanies postnatal repair. This may explain the lack of maxillary growth restriction after in utero cleft lip repair.
AB - This study examines the histologic and biochemical features of wound healing in a cleft lip model in the mid-third-trimester fetal rabbit. At days 1, 2, and 4 after the procedure, control, unrepaired, and repaired fetal heads were obtained, sectioned, and stained for histologic examination. The localization of hyaluronic acid in the wound was documented using a cartilage-derived hyaluronic acid-binding protein. In both repaired and unrepaired wounds, the fetal cleft healed without inflammatory cell infiltration or scar formation. Six months after birth, the repaired cleft showed complete regeneration of muscle across the wound and the collagen fibers were of normal density and orientation. Decreased hyaluronic acid deposition was observed in unrepaired clefts as compared with adjacent tissue; no such difference was detected in repaired clefts. Our findings support the hypothesis that a cleft lip repaired in utero heals without the scarring that accompanies postnatal repair. This may explain the lack of maxillary growth restriction after in utero cleft lip repair.
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U2 - 10.1016/0278-2391(92)90323-R
DO - 10.1016/0278-2391(92)90323-R
M3 - Article
C2 - 1371807
AN - SCOPUS:0026534570
SN - 0278-2391
VL - 50
SP - 263
EP - 268
JO - Journal of Oral and Maxillofacial Surgery
JF - Journal of Oral and Maxillofacial Surgery
IS - 3
ER -