Flow visualization and flow cytometry with holographic video microscopy

Fook Chiong Cheong; Bo Sun; Remi Dreyfus; Jesse Amato-Grill; Ke Xiao; Lisa Dixon; David G. Grier

doi:10.1117/12.840550

Flow visualization and flow cytometry with holographic video microscopy

Fook Chiong Cheong, Bo Sun, Remi Dreyfus, Jesse Amato-Grill, Ke Xiao, Lisa Dixon, David G. Grier

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution, and simultaneously to measure their sizes and refractive indexes. An efficient particle-tracking algorithm automates initial position estimation with sufficient accuracy to enable unattended holographic particle tracking and characterization. In this work, we demonstrated this approach to flow visualization in a microfluidic channel and also to flow cytometry of micrometer-scale colloidal spheres.

Original language	English (US)
Title of host publication	Practical Holography XXIV
Subtitle of host publication	Materials and Applications
DOIs	https://doi.org/10.1117/12.840550
State	Published - 2010
Event	Practical Holography XXIV: Materials and Applications - San Francisco, CA, United States Duration: Jan 24 2010 → Jan 27 2010

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	7619
ISSN (Print)	0277-786X

Other

Other	Practical Holography XXIV: Materials and Applications
Country/Territory	United States
City	San Francisco, CA
Period	1/24/10 → 1/27/10

Keywords

Cytometry
Digital Holography
Particle tracking

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.840550

Cite this

Cheong, FC, Sun, B, Dreyfus, R, Amato-Grill, J, Xiao, K, Dixon, L & Grier, DG 2010, Flow visualization and flow cytometry with holographic video microscopy. in Practical Holography XXIV: Materials and Applications., 761905, Proceedings of SPIE - The International Society for Optical Engineering, vol. 7619, Practical Holography XXIV: Materials and Applications, San Francisco, CA, United States, 1/24/10. https://doi.org/10.1117/12.840550

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series = "Proceedings of SPIE - The International Society for Optical Engineering",

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AU - Cheong, Fook Chiong

AU - Sun, Bo

AU - Dreyfus, Remi

AU - Amato-Grill, Jesse

AU - Xiao, Ke

AU - Dixon, Lisa

AU - Grier, David G.

PY - 2010

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AB - The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution, and simultaneously to measure their sizes and refractive indexes. An efficient particle-tracking algorithm automates initial position estimation with sufficient accuracy to enable unattended holographic particle tracking and characterization. In this work, we demonstrated this approach to flow visualization in a microfluidic channel and also to flow cytometry of micrometer-scale colloidal spheres.

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KW - Digital Holography

KW - Particle tracking

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M3 - Conference contribution

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T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Practical Holography XXIV

T2 - Practical Holography XXIV: Materials and Applications

Y2 - 24 January 2010 through 27 January 2010

ER -

Flow visualization and flow cytometry with holographic video microscopy

Abstract

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Keywords

ASJC Scopus subject areas

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