Flow visualization and flow cytometry with holographic video microscopy

Fook Chiong Cheong, Bo Sun, Remi Dreyfus, Jesse Amato-Grill, Ke Xiao, Lisa Dixon, David G. Grier

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution, and simultaneously to measure their sizes and refractive indexes. An efficient particle-tracking algorithm automates initial position estimation with sufficient accuracy to enable unattended holographic particle tracking and characterization. In this work, we demonstrated this approach to flow visualization in a microfluidic channel and also to flow cytometry of micrometer-scale colloidal spheres.

    Original languageEnglish (US)
    Title of host publicationPractical Holography XXIV
    Subtitle of host publicationMaterials and Applications
    DOIs
    StatePublished - 2010
    EventPractical Holography XXIV: Materials and Applications - San Francisco, CA, United States
    Duration: Jan 24 2010Jan 27 2010

    Publication series

    NameProceedings of SPIE - The International Society for Optical Engineering
    Volume7619
    ISSN (Print)0277-786X

    Other

    OtherPractical Holography XXIV: Materials and Applications
    CountryUnited States
    CitySan Francisco, CA
    Period1/24/101/27/10

    Keywords

    • Cytometry
    • Digital Holography
    • Particle tracking

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics
    • Computer Science Applications
    • Applied Mathematics
    • Electrical and Electronic Engineering

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