Fluidic and air-stable supported lipid bilayer and cell-mimicking microarrays

Yang Deng, Yini Wang, Bryan Holtz, Jingyi Li, Nathan Traaseth, Gianluigi Veglia, Benjamin J. Stottrup, Robert Elde, Duanqing Pei, Athena Guo, X. Y. Zhu

Research output: Contribution to journalArticle

Abstract

As drug delivery, therapy, and medical imaging are becoming increasingly cell-specific, there is a critical need for high fidelity and high-throughput screening methods for cell surface interactions. Cell membrane-mimicking surfaces, i.e., supported lipid bilayers (SLBs), are currently not sufficiently robust to meet this need. Here we describe a method of forming fluidic and air-stable SLBs through tethered and dispersed cholesterol groups incorporated into the bottom leaflet. Achieving air stability allows us to easily fabricate SLB microarrays from direct robotic spotting of vesicle solutions. We demonstrate their application as cell membrane-mimicking microarrays by reconstituting peripheral as well as integral membrane components that can be recognized by their respective targets. These demonstrations establish the viability of the fluidic and air-stable SLB platform for generating content microarrays in high throughput studies, e.g., the screening of drugs and nanomedicine targeting cell surface receptors.

Original languageEnglish (US)
Pages (from-to)6267-6271
Number of pages5
JournalJournal of the American Chemical Society
Volume130
Issue number19
DOIs
StatePublished - May 14 2008

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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    Deng, Y., Wang, Y., Holtz, B., Li, J., Traaseth, N., Veglia, G., Stottrup, B. J., Elde, R., Pei, D., Guo, A., & Zhu, X. Y. (2008). Fluidic and air-stable supported lipid bilayer and cell-mimicking microarrays. Journal of the American Chemical Society, 130(19), 6267-6271. https://doi.org/10.1021/ja800049f