TY - GEN
T1 - FluidTIP for High-Resolution Imaging and Enhanced Micro- and Nano-Scale Biomanipulations in Atomic Force Microscopy
AU - Glia, Ayoub
AU - Deliorman, Muhammedin
AU - Qasaimeh, Mohammad A.
N1 - Publisher Copyright:
© 2023 IEEE.
PY - 2023
Y1 - 2023
N2 - Here, we present the development of a polymeric atomic force microscopy (AFM) probe, named FluidTIP, which features embedded fluidic micro channels and apertures at its tip end. FluidTIP is fabricated using two-photon polymerization, which allows for the design of complex 3D geometries and shapes and integration with commercial AFM systems. The primary design of the FluidTIP includes a single fluidic channel and aperture for fluid delivery or retrieval, while the advanced design includes dual channels and apertures for simultaneous fluid delivery and retrieval. Parametric characterization is well-aligned with experimental validation, confirming the capabilities of the FluidTIP for high-resolution imaging and enhanced micro- and nano-scale biomanipulations in AFM. By combining AFM imaging and force measurements with the ability to manipulate fluids, FluidTIP offers versatile tool for investigating cells with high sensitivity. This advancement in the AFM fluidic cantilever technology opens up new possibilities for investigating the behaviour and interactions of mammalian cells upon delivery or retrieval of biological content.
AB - Here, we present the development of a polymeric atomic force microscopy (AFM) probe, named FluidTIP, which features embedded fluidic micro channels and apertures at its tip end. FluidTIP is fabricated using two-photon polymerization, which allows for the design of complex 3D geometries and shapes and integration with commercial AFM systems. The primary design of the FluidTIP includes a single fluidic channel and aperture for fluid delivery or retrieval, while the advanced design includes dual channels and apertures for simultaneous fluid delivery and retrieval. Parametric characterization is well-aligned with experimental validation, confirming the capabilities of the FluidTIP for high-resolution imaging and enhanced micro- and nano-scale biomanipulations in AFM. By combining AFM imaging and force measurements with the ability to manipulate fluids, FluidTIP offers versatile tool for investigating cells with high sensitivity. This advancement in the AFM fluidic cantilever technology opens up new possibilities for investigating the behaviour and interactions of mammalian cells upon delivery or retrieval of biological content.
KW - Atomic force microscopy
KW - Fluidic cantilever
KW - Single-cell manipulation
KW - Two-photon polymerization
UR - http://www.scopus.com/inward/record.url?scp=85178085235&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85178085235&partnerID=8YFLogxK
U2 - 10.1109/MARSS58567.2023.10294147
DO - 10.1109/MARSS58567.2023.10294147
M3 - Conference contribution
AN - SCOPUS:85178085235
T3 - Proceedings of MARSS 2023 - 6th International Conference on Manipulation, Automation, and Robotics at Small Scales
BT - Proceedings of MARSS 2023 - 6th International Conference on Manipulation, Automation, and Robotics at Small Scales
A2 - Haliyo, Sinan
A2 - Boudaoud, Mokrane
A2 - Qasaimeh, Mohammad A.
A2 - Fatikow, Sergej
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 6th International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2023
Y2 - 9 October 2023 through 13 October 2023
ER -