The degree of polarization of fluorescence (P) of chlorophyll a in vivo has been measured using suspensions of either randomly oriented or magnetically oriented spinach chloroplasts. In the latter case the viewing direction was perpendicular to the plane of the oriented photosynthetic membranes. P decreases from 3.5% (unoriented) to 1.5% when the chloroplasts are oriented in a 13 Kgauss magnetic field. This decrease can be explained by the anisotropic optical properties of the membranes. The residual 1.5% polarization is shown to reflect the depolarization by energy transfer. Similar effects have been observed with Chlorella.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Oct 15 1973|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology