TY - JOUR
T1 - Fluorescence hplc methods for detecting benzo[a]pyrene-7,8-dihydrodiol 9,10-oxide-deoxyadenosine adducts in enzyme-digests of modified dna
T2 - Improved sensitivity
AU - Chen, Junxin
AU - Macleod, Michael C.
AU - Zhao, Rushen
AU - E.geacintov, Nicholas
N1 - Funding Information:
The portion of this work carried out at New York University was supported by the Department of Energy, Office of Health and Environmental Research, Grant DE-FGO2-86ER60405; the portion carried out at the University of Texas M.D. Anderson Cancer Center was supported by a grant from the National Cancer Institute (CA-35581).
PY - 1993/5
Y1 - 1993/5
N2 - The fluorescence of mononucleoside adducts derived from the binding of anti-7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetra-hydrobenzo[a]pyrene (BPDE I) to N6-deoxyadenosine (BPDE-dA adducts) is 10-100 times stronger (depending on the methanol/water solvent composition) than the fluorescence of adducts derived from the binding of this diol epoxide derivative to N2-deoxyguanosine. It is shown here that these fluorescence characteristics can be used to quantitate the relatively low yields of BPDE-dA adducts by fluorescence detection when BPDE-modified DNA is subjected to enzymatic degradation to the mononucleoside levels, followed by HPLC analysis of the digests.
AB - The fluorescence of mononucleoside adducts derived from the binding of anti-7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetra-hydrobenzo[a]pyrene (BPDE I) to N6-deoxyadenosine (BPDE-dA adducts) is 10-100 times stronger (depending on the methanol/water solvent composition) than the fluorescence of adducts derived from the binding of this diol epoxide derivative to N2-deoxyguanosine. It is shown here that these fluorescence characteristics can be used to quantitate the relatively low yields of BPDE-dA adducts by fluorescence detection when BPDE-modified DNA is subjected to enzymatic degradation to the mononucleoside levels, followed by HPLC analysis of the digests.
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U2 - 10.1093/carcin/14.5.1049
DO - 10.1093/carcin/14.5.1049
M3 - Article
C2 - 8504464
AN - SCOPUS:0027276369
SN - 0143-3334
VL - 14
SP - 1049
EP - 1051
JO - Carcinogenesis
JF - Carcinogenesis
IS - 5
ER -