Fluorescence hplc methods for detecting benzo[a]pyrene-7,8-dihydrodiol 9,10-oxide-deoxyadenosine adducts in enzyme-digests of modified dna: Improved sensitivity

Junxin Chen, Michael C. Macleod, Rushen Zhao, Nicholas E.geacintov

Research output: Contribution to journalArticlepeer-review

Abstract

The fluorescence of mononucleoside adducts derived from the binding of anti-7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetra-hydrobenzo[a]pyrene (BPDE I) to N6-deoxyadenosine (BPDE-dA adducts) is 10-100 times stronger (depending on the methanol/water solvent composition) than the fluorescence of adducts derived from the binding of this diol epoxide derivative to N2-deoxyguanosine. It is shown here that these fluorescence characteristics can be used to quantitate the relatively low yields of BPDE-dA adducts by fluorescence detection when BPDE-modified DNA is subjected to enzymatic degradation to the mononucleoside levels, followed by HPLC analysis of the digests.

Original languageEnglish (US)
Pages (from-to)1049-1051
Number of pages3
JournalCarcinogenesis
Volume14
Issue number5
DOIs
StatePublished - May 1993

ASJC Scopus subject areas

  • Cancer Research

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