TY - JOUR
T1 - Fluorescence properties of a benzo(a)pyrene 7,8 dihydrodiol 9,10-oxide-DNA adduct. Conformation and effects of intermolecular DNA interactions
AU - Prusik, Thaddeus
AU - Geacintov, Nicholas E.
N1 - Funding Information:
We gratefully acknowledge a gift of 7,8-dihydrodiol 9,10-epoxy benzo(a)-pyrene from Dr. R.G. Harvey. We thank Dr. V. Ibanez for preparing the covalent DNA adducts. This investigation was supported by contracts from the U.S. Department of Energy (EP-78-S-02-4959 and E(ll-1)2386).
PY - 1979/6/13
Y1 - 1979/6/13
N2 - The pyrene-like fluorescence of the covalent benzo(a)pyrene diol-epoxide-DNA complex prepared by reacting 7,8,-dihydrodiol 9,10-epoxy benzo(a)pyrene (BPDE) with DNA in aqueous solution in vitro, has been investigated. It is shown that this fluorescence is sensitive to molecular oxygen, to the concentration of native DNA and to the ionic strength (KCl concentration), but is insensitive to the concentration of denatured DNA. These effects are related to the conformation of the pyrene-like chromophore of BPDE. Most of the fluorescence of a dilute solution of the DNA-bound benzo(a)pyrene derivative originates from binding sites in which the pyrene moiety is not intercalated between the DNA base pairs, but is located on the outside of the DNA double helix.
AB - The pyrene-like fluorescence of the covalent benzo(a)pyrene diol-epoxide-DNA complex prepared by reacting 7,8,-dihydrodiol 9,10-epoxy benzo(a)pyrene (BPDE) with DNA in aqueous solution in vitro, has been investigated. It is shown that this fluorescence is sensitive to molecular oxygen, to the concentration of native DNA and to the ionic strength (KCl concentration), but is insensitive to the concentration of denatured DNA. These effects are related to the conformation of the pyrene-like chromophore of BPDE. Most of the fluorescence of a dilute solution of the DNA-bound benzo(a)pyrene derivative originates from binding sites in which the pyrene moiety is not intercalated between the DNA base pairs, but is located on the outside of the DNA double helix.
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U2 - 10.1016/0006-291X(79)91476-1
DO - 10.1016/0006-291X(79)91476-1
M3 - Article
C2 - 572681
AN - SCOPUS:0018428410
SN - 0006-291X
VL - 88
SP - 782
EP - 790
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -