Fluoride exposure alters Ca2+ signaling and mitochondrial function in enamel cells

Francisco J. Aulestia; Johnny Groeling; Guilherme H.S. Bomfim; Veronica Costiniti; Vinu Manikandan; Ariya Chaloemtoem; Axel R. Concepcion; Yi Li; Larry E. Wagner; Youssef Idaghdour; David I. Yule; Rodrigo S. Lacruz

doi:10.1126/scisignal.aay0086

Fluoride exposure alters Ca²⁺ signaling and mitochondrial function in enamel cells

Francisco J. Aulestia, Johnny Groeling, Guilherme H.S. Bomfim, Veronica Costiniti, Vinu Manikandan, Ariya Chaloemtoem, Axel R. Concepcion, Yi Li, Larry E. Wagner, Youssef Idaghdour, David I. Yule, Rodrigo S. Lacruz

Research output: Contribution to journal › Article › peer-review

Abstract

Fluoride ions are highly reactive, and their incorporation in forming dental enamel at low concentrations promotes mineralization. In contrast, excessive fluoride intake causes dental fluorosis, visually recognizable enamel defects that can increase the risk of caries. To investigate the molecular bases of dental fluorosis, we analyzed the effects of fluoride exposure in enamel cells to assess its impact on Ca²⁺ signaling. Primary enamel cells and an enamel cell line (LS8) exposed to fluoride showed decreased internal Ca²⁺ stores and store-operated Ca²⁺ entry (SOCE). RNA-sequencing analysis revealed changes in gene expression suggestive of endoplasmic reticulum (ER) stress in fluoride-treated LS8 cells. Fluoride exposure did not alter Ca²⁺ homeostasis or increase the expression of ER stress–associated genes in HEK-293 cells. In enamel cells, fluoride exposure affected the functioning of the ER-localized Ca²⁺ channel IP₃R and the activity of the sarco-endoplasmic reticulum Ca²⁺-ATPase (SERCA) pump during Ca²⁺ refilling of the ER. Fluoride negatively affected mitochondrial respiration, elicited mitochondrial membrane depolarization, and disrupted mitochondrial morphology. Together, these data provide a potential mechanism underlying dental fluorosis.

Original language	English (US)
Article number	eaay0086
Journal	Science signaling
Volume	13
Issue number	619
DOIs	https://doi.org/10.1126/scisignal.aay0086
State	Published - Feb 18 2020

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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Fluoride exposure alters Ca²⁺ signaling and mitochondrial function in enamel cells. / Aulestia, Francisco J.; Groeling, Johnny; Bomfim, Guilherme H.S.; Costiniti, Veronica; Manikandan, Vinu; Chaloemtoem, Ariya; Concepcion, Axel R.; Li, Yi; Wagner, Larry E.; Idaghdour, Youssef; Yule, David I.; Lacruz, Rodrigo S.

In: Science signaling, Vol. 13, No. 619, eaay0086, 18.02.2020.

Research output: Contribution to journal › Article › peer-review

Aulestia, Francisco J. ; Groeling, Johnny ; Bomfim, Guilherme H.S. ; Costiniti, Veronica ; Manikandan, Vinu ; Chaloemtoem, Ariya ; Concepcion, Axel R. ; Li, Yi ; Wagner, Larry E. ; Idaghdour, Youssef ; Yule, David I. ; Lacruz, Rodrigo S. / Fluoride exposure alters Ca²⁺ signaling and mitochondrial function in enamel cells. In: Science signaling. 2020 ; Vol. 13, No. 619.

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title = "Fluoride exposure alters Ca2+ signaling and mitochondrial function in enamel cells",

abstract = "Fluoride ions are highly reactive, and their incorporation in forming dental enamel at low concentrations promotes mineralization. In contrast, excessive fluoride intake causes dental fluorosis, visually recognizable enamel defects that can increase the risk of caries. To investigate the molecular bases of dental fluorosis, we analyzed the effects of fluoride exposure in enamel cells to assess its impact on Ca2+ signaling. Primary enamel cells and an enamel cell line (LS8) exposed to fluoride showed decreased internal Ca2+ stores and store-operated Ca2+ entry (SOCE). RNA-sequencing analysis revealed changes in gene expression suggestive of endoplasmic reticulum (ER) stress in fluoride-treated LS8 cells. Fluoride exposure did not alter Ca2+ homeostasis or increase the expression of ER stress–associated genes in HEK-293 cells. In enamel cells, fluoride exposure affected the functioning of the ER-localized Ca2+ channel IP3R and the activity of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) pump during Ca2+ refilling of the ER. Fluoride negatively affected mitochondrial respiration, elicited mitochondrial membrane depolarization, and disrupted mitochondrial morphology. Together, these data provide a potential mechanism underlying dental fluorosis.",

author = "Aulestia, {Francisco J.} and Johnny Groeling and Bomfim, {Guilherme H.S.} and Veronica Costiniti and Vinu Manikandan and Ariya Chaloemtoem and Concepcion, {Axel R.} and Yi Li and Wagner, {Larry E.} and Youssef Idaghdour and Yule, {David I.} and Lacruz, {Rodrigo S.}",

note = "Funding Information: This work was funded by the National Institute of Dental and Craniofacial Research (NIH/NIDCR) (R01DE025639 and R01DE027679 to R.S.L. and R01DE014756 to D.I.Y.). Y.I. was supported by New York University Abu Dhabi research grant AD105. The NYU Genome Technology Center is partially supported by Cancer Center Support grant P30CA016087 at the Laura and Isaac Perlmutter Cancer Center. Publisher Copyright: Copyright {\textcopyright} 2020 The Authors, some rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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doi = "10.1126/scisignal.aay0086",

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AU - Aulestia, Francisco J.

AU - Groeling, Johnny

AU - Bomfim, Guilherme H.S.

AU - Costiniti, Veronica

AU - Manikandan, Vinu

AU - Chaloemtoem, Ariya

AU - Concepcion, Axel R.

AU - Li, Yi

AU - Wagner, Larry E.

AU - Idaghdour, Youssef

AU - Yule, David I.

AU - Lacruz, Rodrigo S.

N1 - Funding Information: This work was funded by the National Institute of Dental and Craniofacial Research (NIH/NIDCR) (R01DE025639 and R01DE027679 to R.S.L. and R01DE014756 to D.I.Y.). Y.I. was supported by New York University Abu Dhabi research grant AD105. The NYU Genome Technology Center is partially supported by Cancer Center Support grant P30CA016087 at the Laura and Isaac Perlmutter Cancer Center. Publisher Copyright: Copyright © 2020 The Authors, some rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/2/18

Y1 - 2020/2/18

N2 - Fluoride ions are highly reactive, and their incorporation in forming dental enamel at low concentrations promotes mineralization. In contrast, excessive fluoride intake causes dental fluorosis, visually recognizable enamel defects that can increase the risk of caries. To investigate the molecular bases of dental fluorosis, we analyzed the effects of fluoride exposure in enamel cells to assess its impact on Ca2+ signaling. Primary enamel cells and an enamel cell line (LS8) exposed to fluoride showed decreased internal Ca2+ stores and store-operated Ca2+ entry (SOCE). RNA-sequencing analysis revealed changes in gene expression suggestive of endoplasmic reticulum (ER) stress in fluoride-treated LS8 cells. Fluoride exposure did not alter Ca2+ homeostasis or increase the expression of ER stress–associated genes in HEK-293 cells. In enamel cells, fluoride exposure affected the functioning of the ER-localized Ca2+ channel IP3R and the activity of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) pump during Ca2+ refilling of the ER. Fluoride negatively affected mitochondrial respiration, elicited mitochondrial membrane depolarization, and disrupted mitochondrial morphology. Together, these data provide a potential mechanism underlying dental fluorosis.

AB - Fluoride ions are highly reactive, and their incorporation in forming dental enamel at low concentrations promotes mineralization. In contrast, excessive fluoride intake causes dental fluorosis, visually recognizable enamel defects that can increase the risk of caries. To investigate the molecular bases of dental fluorosis, we analyzed the effects of fluoride exposure in enamel cells to assess its impact on Ca2+ signaling. Primary enamel cells and an enamel cell line (LS8) exposed to fluoride showed decreased internal Ca2+ stores and store-operated Ca2+ entry (SOCE). RNA-sequencing analysis revealed changes in gene expression suggestive of endoplasmic reticulum (ER) stress in fluoride-treated LS8 cells. Fluoride exposure did not alter Ca2+ homeostasis or increase the expression of ER stress–associated genes in HEK-293 cells. In enamel cells, fluoride exposure affected the functioning of the ER-localized Ca2+ channel IP3R and the activity of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) pump during Ca2+ refilling of the ER. Fluoride negatively affected mitochondrial respiration, elicited mitochondrial membrane depolarization, and disrupted mitochondrial morphology. Together, these data provide a potential mechanism underlying dental fluorosis.

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