Force generation in RNA polymerase

Hong Yun Wang; Tim Elston; Alexander Mogilner; George Oster

doi:10.1016/S0006-3495(98)77834-8

Force generation in RNA polymerase

Hong Yun Wang, Tim Elston, Alexander Mogilner, George Oster

Research output: Contribution to journal › Article › peer-review

Abstract

RNA polymerase (RNAP) is a processive molecular motor capable of generating forces of 25-30 pN, far in excess of any other known ATPase. This force derives from the hydrolysis free energy of nucleotides as they are incorporated into the growing RNA chain. The velocity of procession is limited by the rate of pyrophosphate release. Here we demonstrate how nucleotide triphosphate binding free energy can rectify the diffusion of RNAP, and show that this is sufficient to account for the quantitative features of the measured load-velocity curve. Predictions are made for the effect of changing pyrophosphate and nucleotide concentrations and for the statistical behavior of the system.

Original language	English (US)
Pages (from-to)	1186-1202
Number of pages	17
Journal	Biophysical journal
Volume	74
Issue number	3
DOIs	https://doi.org/10.1016/S0006-3495(98)77834-8
State	Published - Mar 1998

ASJC Scopus subject areas

Biophysics

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10.1016/S0006-3495(98)77834-8

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title = "Force generation in RNA polymerase",

abstract = "RNA polymerase (RNAP) is a processive molecular motor capable of generating forces of 25-30 pN, far in excess of any other known ATPase. This force derives from the hydrolysis free energy of nucleotides as they are incorporated into the growing RNA chain. The velocity of procession is limited by the rate of pyrophosphate release. Here we demonstrate how nucleotide triphosphate binding free energy can rectify the diffusion of RNAP, and show that this is sufficient to account for the quantitative features of the measured load-velocity curve. Predictions are made for the effect of changing pyrophosphate and nucleotide concentrations and for the statistical behavior of the system.",

author = "Wang, {Hong Yun} and Tim Elston and Alexander Mogilner and George Oster",

note = "Funding Information: H-YW was supported by a postdoctoral fellowship from the National Energy Research Scientific Computing Center, TE by postdoctoral support from Los Alamos National Laboratory, and GO by National Science Foundation grant DMS 9220719. ",

year = "1998",

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doi = "10.1016/S0006-3495(98)77834-8",

language = "English (US)",

volume = "74",

pages = "1186--1202",

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TY - JOUR

T1 - Force generation in RNA polymerase

AU - Wang, Hong Yun

AU - Elston, Tim

AU - Mogilner, Alexander

AU - Oster, George

N1 - Funding Information: H-YW was supported by a postdoctoral fellowship from the National Energy Research Scientific Computing Center, TE by postdoctoral support from Los Alamos National Laboratory, and GO by National Science Foundation grant DMS 9220719.

PY - 1998/3

Y1 - 1998/3

N2 - RNA polymerase (RNAP) is a processive molecular motor capable of generating forces of 25-30 pN, far in excess of any other known ATPase. This force derives from the hydrolysis free energy of nucleotides as they are incorporated into the growing RNA chain. The velocity of procession is limited by the rate of pyrophosphate release. Here we demonstrate how nucleotide triphosphate binding free energy can rectify the diffusion of RNAP, and show that this is sufficient to account for the quantitative features of the measured load-velocity curve. Predictions are made for the effect of changing pyrophosphate and nucleotide concentrations and for the statistical behavior of the system.

AB - RNA polymerase (RNAP) is a processive molecular motor capable of generating forces of 25-30 pN, far in excess of any other known ATPase. This force derives from the hydrolysis free energy of nucleotides as they are incorporated into the growing RNA chain. The velocity of procession is limited by the rate of pyrophosphate release. Here we demonstrate how nucleotide triphosphate binding free energy can rectify the diffusion of RNAP, and show that this is sufficient to account for the quantitative features of the measured load-velocity curve. Predictions are made for the effect of changing pyrophosphate and nucleotide concentrations and for the statistical behavior of the system.

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Force generation in RNA polymerase

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