Force measurements between bacteria and poly(ethylene glycol)-coated surfaces

Anneta Razatos; Yea Ling Ong; Fabienne Boulay; Donald L. Elbert; Jeffrey A. Hubbell; Mukul M. Sharma; George Georgiou

doi:10.1021/la000818y

Force measurements between bacteria and poly(ethylene glycol)-coated surfaces

Anneta Razatos, Yea Ling Ong, Fabienne Boulay, Donald L. Elbert, Jeffrey A. Hubbell, Mukul M. Sharma, George Georgiou

Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

The atomic force microscope (AFM) was used to directly measure the forces of interaction between E. coli D21 bacteria and hydrophilic glass or hydrophobic N-octadecyltrichlorosilane (OTS)-treated glass substrates coated with the block copolymers, poly(ethylene glycol) (PEG)-lysine dendron or Pluronic F127 surfactant, respectively. Short-range repulsive interactions between bacterial cells and substrates coated with the block copolymers were detected by the AFM over distances of separation comparable to the extended length of the PEG polymer chains. In contrast, glass and OTS-treated glass devoid of PEG-lysine dendron or Pluronic F127 exerted long-range attractive forces on E. coli D21 bacteria. Thus, polymeric brush layers appear to not only block the long-range attractive forces of interaction between bacteria and substrates but also introduce repulsive steric effects.

Original language	English (US)
Pages (from-to)	9155-9158
Number of pages	4
Journal	Langmuir
Volume	16
Issue number	24
DOIs	https://doi.org/10.1021/la000818y
State	Published - Nov 28 2000

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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10.1021/la000818y

Cite this

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title = "Force measurements between bacteria and poly(ethylene glycol)-coated surfaces",

abstract = "The atomic force microscope (AFM) was used to directly measure the forces of interaction between E. coli D21 bacteria and hydrophilic glass or hydrophobic N-octadecyltrichlorosilane (OTS)-treated glass substrates coated with the block copolymers, poly(ethylene glycol) (PEG)-lysine dendron or Pluronic F127 surfactant, respectively. Short-range repulsive interactions between bacterial cells and substrates coated with the block copolymers were detected by the AFM over distances of separation comparable to the extended length of the PEG polymer chains. In contrast, glass and OTS-treated glass devoid of PEG-lysine dendron or Pluronic F127 exerted long-range attractive forces on E. coli D21 bacteria. Thus, polymeric brush layers appear to not only block the long-range attractive forces of interaction between bacteria and substrates but also introduce repulsive steric effects.",

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TY - JOUR

T1 - Force measurements between bacteria and poly(ethylene glycol)-coated surfaces

AU - Razatos, Anneta

AU - Ong, Yea Ling

AU - Boulay, Fabienne

AU - Elbert, Donald L.

AU - Hubbell, Jeffrey A.

AU - Sharma, Mukul M.

AU - Georgiou, George

PY - 2000/11/28

Y1 - 2000/11/28

N2 - The atomic force microscope (AFM) was used to directly measure the forces of interaction between E. coli D21 bacteria and hydrophilic glass or hydrophobic N-octadecyltrichlorosilane (OTS)-treated glass substrates coated with the block copolymers, poly(ethylene glycol) (PEG)-lysine dendron or Pluronic F127 surfactant, respectively. Short-range repulsive interactions between bacterial cells and substrates coated with the block copolymers were detected by the AFM over distances of separation comparable to the extended length of the PEG polymer chains. In contrast, glass and OTS-treated glass devoid of PEG-lysine dendron or Pluronic F127 exerted long-range attractive forces on E. coli D21 bacteria. Thus, polymeric brush layers appear to not only block the long-range attractive forces of interaction between bacteria and substrates but also introduce repulsive steric effects.

AB - The atomic force microscope (AFM) was used to directly measure the forces of interaction between E. coli D21 bacteria and hydrophilic glass or hydrophobic N-octadecyltrichlorosilane (OTS)-treated glass substrates coated with the block copolymers, poly(ethylene glycol) (PEG)-lysine dendron or Pluronic F127 surfactant, respectively. Short-range repulsive interactions between bacterial cells and substrates coated with the block copolymers were detected by the AFM over distances of separation comparable to the extended length of the PEG polymer chains. In contrast, glass and OTS-treated glass devoid of PEG-lysine dendron or Pluronic F127 exerted long-range attractive forces on E. coli D21 bacteria. Thus, polymeric brush layers appear to not only block the long-range attractive forces of interaction between bacteria and substrates but also introduce repulsive steric effects.

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Force measurements between bacteria and poly(ethylene glycol)-coated surfaces

Abstract

ASJC Scopus subject areas

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