Force Spectroscopy with a Small Dithering of AFM Tip: A Method of Direct and Continuous Measurement of the Spring Constant of Single Molecules and Molecular Complexes

Lilia A. Chtcheglova; George T. Shubeita; Sergey K. Sekatskii; Giovanni Dietler

doi:10.1016/S0006-3495(04)74192-2

Force Spectroscopy with a Small Dithering of AFM Tip: A Method of Direct and Continuous Measurement of the Spring Constant of Single Molecules and Molecular Complexes

Lilia A. Chtcheglova, George T. Shubeita, Sergey K. Sekatskii, Giovanni Dietler

Physics

Research output: Contribution to journal › Article › peer-review

Abstract

A new method of direct and continuous measurement of the spring constant of single molecule or molecular complex is elaborated. To that end the standard force spectroscopy technique with functionalized tips and samples is combined with a small dithering of the tip. The change of the dithering amplitude as a function of the pulling force is measured to extract the spring constant of the complex. The potentialities of this method are illustrated for the experiments with single bovine serum albumin-its polyclonal antibody (Ab-BSA) and fibrinogen-fibrinogen complexes.

Original language	English (US)
Pages (from-to)	1177-1184
Number of pages	8
Journal	Biophysical journal
Volume	86
Issue number	2
DOIs	https://doi.org/10.1016/S0006-3495(04)74192-2
State	Published - Feb 2004

ASJC Scopus subject areas

Biophysics

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10.1016/S0006-3495(04)74192-2

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Force Spectroscopy with a Small Dithering of AFM Tip: A Method of Direct and Continuous Measurement of the Spring Constant of Single Molecules and Molecular Complexes. / Chtcheglova, Lilia A.; Shubeita, George T.; Sekatskii, Sergey K. et al.
In: Biophysical journal, Vol. 86, No. 2, 02.2004, p. 1177-1184.

Research output: Contribution to journal › Article › peer-review

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abstract = "A new method of direct and continuous measurement of the spring constant of single molecule or molecular complex is elaborated. To that end the standard force spectroscopy technique with functionalized tips and samples is combined with a small dithering of the tip. The change of the dithering amplitude as a function of the pulling force is measured to extract the spring constant of the complex. The potentialities of this method are illustrated for the experiments with single bovine serum albumin-its polyclonal antibody (Ab-BSA) and fibrinogen-fibrinogen complexes.",

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note = "Funding Information: This study was supported by the Swiss National Science Foundation (grant Nos. 2000-065160.01, 2000-61751.00, and 7SUPJ062202).",

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AU - Sekatskii, Sergey K.

AU - Dietler, Giovanni

N1 - Funding Information: This study was supported by the Swiss National Science Foundation (grant Nos. 2000-065160.01, 2000-61751.00, and 7SUPJ062202).

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Force Spectroscopy with a Small Dithering of AFM Tip: A Method of Direct and Continuous Measurement of the Spring Constant of Single Molecules and Molecular Complexes

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