Abstract
The chromatin fiber undergoes significant structural changes during the cell's life cycle to modulate DNA accessibility. Detailed mechanisms of such structural transformations of chromatin fibers as affected by various internal and external conditions such as the ionic conditions of the medium, the linker DNA length, and the presence of linker histones, constitute an open challenge. Here we utilize Monte Carlo (MC) simulations of a coarse grained model of chromatin with nonuniform linker DNA lengths as found in vivo to help explain some aspects of this challenge. We investigate the unfolding mechanisms of chromatin fibers with alternating linker lengths of 26-62bp and 44-79bp using a series of end-to-end stretching trajectories with and without linker histones and compare results to uniform-linker-length fibers. We find that linker histones increase overall resistance of nonuniform fibers and lead to fiber unfolding with superbeads-on-a-string cluster transitions. Chromatin fibers with nonuniform linker DNA lengths display a more complex, multi-step yet smoother process of unfolding compared to their uniform counterparts, likely due to the existence of a more continuous range of nucleosome-nucleosome interactions. This finding echoes the theme that some heterogeneity in fiber component is biologically advantageous.
Original language | English (US) |
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Article number | 064113 |
Journal | Journal of Physics Condensed Matter |
Volume | 27 |
Issue number | 6 |
DOIs | |
State | Published - Feb 18 2015 |
Keywords
- Monte Carlo
- alternating linker DNA length
- chromatin
- coarse-grained modeling
- force pulling
- nonuniform NRL
ASJC Scopus subject areas
- Materials Science(all)
- Condensed Matter Physics