Fourth rank immobile nucleic acid junctions

Neville R. Kallenbach, Rong Ine Ma, A. Joshua Wand, Gerrit H. Veeneman, Jacques H. Van Boom, Nadrian C. Seeman

Research output: Contribution to journalArticlepeer-review

Abstract

An immobile nucleic acid junction composed of four dodecanucleotides has been designed according to principles of minimum symmetry aided by equilibrium calculations, and has been synthesized by automated phosphotriester techniques. We can demonstrate its tetrameric character and its 1: 1: 1: 1 stoichiometry by gel electrophoresis. Thermal denaturation monitored by ultraviolet hyperchromism indicates that the complex is stable relative to its component arms. High resolution NMR spectroscopy suggests that this junction exists in more than one conformer at room temperature. The data from this junction are compared with the data from a similar junction composed of four hexadecanucleotides.

Original languageEnglish (US)
Pages (from-to)159-168
Number of pages10
JournalJournal of Biomolecular Structure and Dynamics
Volume1
Issue number1
DOIs
StatePublished - Oct 1983

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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