TY - JOUR
T1 - Fractionation of poly(ethylene glycol) by high osmotic pressure chromatography
AU - Matsuyama, Shigetomo
AU - Nakahara, Hisae
AU - Takeuchi, Kazuhiko
AU - Nagahata, Ritsuko
AU - Kinugasa, Shinichi
AU - Teraoka, Iwao
PY - 2000
Y1 - 2000
N2 - Poly(ethylene glycol) (PEG) was fractionated by high osmotic pressure chromatography (HOPC) with water and 1,4-dioxane as eluents. Using dimethyloctylchlorosilane (C8)-modified controlled-pore glass (CPG) with 1,4-dioxane as eluent, high molar mass (MM) PEG components were fractionated. The average MM of the fractionated PEG samples strongly depends on the eluent and the polarity of column packing materials. PEGs dissolved in water were adsorbed onto the column packing materials more easily compared with 1,4-dioxane. Comparison between simulation and experiments indicated that adsorption of PEG molecules onto the surface of the column packing materials reduces the separation efficiency of HOPC fractionation. By preventing adsorption, PEG components with higher MM and narrow molar mass distribution (MMD) were fractionated even from a commercial PEG with narrow MMD.
AB - Poly(ethylene glycol) (PEG) was fractionated by high osmotic pressure chromatography (HOPC) with water and 1,4-dioxane as eluents. Using dimethyloctylchlorosilane (C8)-modified controlled-pore glass (CPG) with 1,4-dioxane as eluent, high molar mass (MM) PEG components were fractionated. The average MM of the fractionated PEG samples strongly depends on the eluent and the polarity of column packing materials. PEGs dissolved in water were adsorbed onto the column packing materials more easily compared with 1,4-dioxane. Comparison between simulation and experiments indicated that adsorption of PEG molecules onto the surface of the column packing materials reduces the separation efficiency of HOPC fractionation. By preventing adsorption, PEG components with higher MM and narrow molar mass distribution (MMD) were fractionated even from a commercial PEG with narrow MMD.
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U2 - 10.1295/polymj.32.249
DO - 10.1295/polymj.32.249
M3 - Article
AN - SCOPUS:0033904974
SN - 0032-3896
VL - 32
SP - 249
EP - 254
JO - Polymer Journal
JF - Polymer Journal
IS - 3
ER -