Frequent human genomic DNA transduction driven by line-1 retrotransposition

Oxana K. Pickeral, Wojciech Makałowski, Mark S. Boguski, Jef D. Boeke

    Research output: Contribution to journalArticle

    Abstract

    Human L1 retrotransposons can produce DNA transduction events in which unique DNA segments downstream of L1 elements are mobilized as part of aberrant retrotransposition events. That L1s are capable of carrying out such a reaction in tissue culture cells was elegantly demonstrated. Using bioinformatic approaches to analyze the structures of L1 element target site duplications and flanking sequence features, we provide evidence suggesting that ~15% of full-length L1 elements bear evidence of flanking DNA segment transduction. Extrapolating these findings to the 600,000 copies of L1 in the genome, we predict that the amount of DNA transduced by L1 represents ~1% of the genome, a fraction comparable with that occupied by exons.

    Original languageEnglish (US)
    Pages (from-to)411-415
    Number of pages5
    JournalGenome Research
    Volume10
    Issue number4
    DOIs
    StatePublished - Apr 1 2000

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    ASJC Scopus subject areas

    • Genetics
    • Genetics(clinical)

    Cite this

    Pickeral, O. K., Makałowski, W., Boguski, M. S., & Boeke, J. D. (2000). Frequent human genomic DNA transduction driven by line-1 retrotransposition. Genome Research, 10(4), 411-415. https://doi.org/10.1101/gr.10.4.411