Functional analysis of microRNA activity in Brugia malayi

Canhui Liu; Denis Voronin; Catherine B. Poole; Saheed Bachu; Matthew B. Rogers; Jingmin Jin; Elodie Ghedin; Sara Lustigman; Larry A. McReynolds; Thomas R. Unnasch

doi:10.1016/j.ijpara.2015.04.004

Functional analysis of microRNA activity in Brugia malayi

Canhui Liu, Denis Voronin, Catherine B. Poole, Saheed Bachu, Matthew B. Rogers, Jingmin Jin, Elodie Ghedin, Sara Lustigman, Larry A. McReynolds, Thomas R. Unnasch

Research output: Contribution to journal › Article › peer-review

Abstract

The complement of the Brugia malayi microRNA-71 was inserted into the 3' untranslated region of a reporter plasmid, resulting in a decrease in reporter activity. Mutation of the seed sequence restored activity. Insertion of the 3' untranslated regions from two algorithm-predicted putative target genes into the reporter resulted in a similar decrease in activity; mutation of the predicted target sequences restored activity. These experiments demonstrate that B. malayi microRNA targets may be predicted using current algorithms and describe a functional assay to confirm predicted targets.

Original language	English (US)
Pages (from-to)	579-583
Number of pages	5
Journal	International Journal for Parasitology
Volume	45
Issue number	9-10
DOIs	https://doi.org/10.1016/j.ijpara.2015.04.004
State	Published - Aug 1 2015

Keywords

Filariasis
Genetic regulation
MiRNA
Transfection

ASJC Scopus subject areas

Parasitology
Infectious Diseases

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10.1016/j.ijpara.2015.04.004

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title = "Functional analysis of microRNA activity in Brugia malayi",

abstract = "The complement of the Brugia malayi microRNA-71 was inserted into the 3' untranslated region of a reporter plasmid, resulting in a decrease in reporter activity. Mutation of the seed sequence restored activity. Insertion of the 3' untranslated regions from two algorithm-predicted putative target genes into the reporter resulted in a similar decrease in activity; mutation of the predicted target sequences restored activity. These experiments demonstrate that B. malayi microRNA targets may be predicted using current algorithms and describe a functional assay to confirm predicted targets.",

keywords = "Filariasis, Genetic regulation, MiRNA, Transfection",

author = "Canhui Liu and Denis Voronin and Poole, {Catherine B.} and Saheed Bachu and Rogers, {Matthew B.} and Jingmin Jin and Elodie Ghedin and Sara Lustigman and McReynolds, {Larry A.} and Unnasch, {Thomas R.}",

note = "Funding Information: Parasite material used in this study was obtained through the Filariasis Research Reagent Resource Center (FR3), Division of Microbiology and infectious Diseases, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health, USA. This work was supported by a grant from the United States NIAID (Project # 1R56AI101372-01A1) to SL, EG and TRU. Publisher Copyright: {\textcopyright} 2015 Australian Society for Parasitology Inc.",

year = "2015",

month = aug,

day = "1",

doi = "10.1016/j.ijpara.2015.04.004",

language = "English (US)",

volume = "45",

pages = "579--583",

journal = "International Journal for Parasitology",

issn = "0020-7519",

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number = "9-10",

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TY - JOUR

T1 - Functional analysis of microRNA activity in Brugia malayi

AU - Liu, Canhui

AU - Voronin, Denis

AU - Poole, Catherine B.

AU - Bachu, Saheed

AU - Rogers, Matthew B.

AU - Jin, Jingmin

AU - Ghedin, Elodie

AU - Lustigman, Sara

AU - McReynolds, Larry A.

AU - Unnasch, Thomas R.

N1 - Funding Information: Parasite material used in this study was obtained through the Filariasis Research Reagent Resource Center (FR3), Division of Microbiology and infectious Diseases, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health, USA. This work was supported by a grant from the United States NIAID (Project # 1R56AI101372-01A1) to SL, EG and TRU. Publisher Copyright: © 2015 Australian Society for Parasitology Inc.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - The complement of the Brugia malayi microRNA-71 was inserted into the 3' untranslated region of a reporter plasmid, resulting in a decrease in reporter activity. Mutation of the seed sequence restored activity. Insertion of the 3' untranslated regions from two algorithm-predicted putative target genes into the reporter resulted in a similar decrease in activity; mutation of the predicted target sequences restored activity. These experiments demonstrate that B. malayi microRNA targets may be predicted using current algorithms and describe a functional assay to confirm predicted targets.

AB - The complement of the Brugia malayi microRNA-71 was inserted into the 3' untranslated region of a reporter plasmid, resulting in a decrease in reporter activity. Mutation of the seed sequence restored activity. Insertion of the 3' untranslated regions from two algorithm-predicted putative target genes into the reporter resulted in a similar decrease in activity; mutation of the predicted target sequences restored activity. These experiments demonstrate that B. malayi microRNA targets may be predicted using current algorithms and describe a functional assay to confirm predicted targets.

KW - Filariasis

KW - Genetic regulation

KW - MiRNA

KW - Transfection

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DO - 10.1016/j.ijpara.2015.04.004

M3 - Article

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AN - SCOPUS:84937524184

SN - 0020-7519

VL - 45

SP - 579

EP - 583

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 9-10

ER -

Functional analysis of microRNA activity in Brugia malayi

Abstract

Keywords

ASJC Scopus subject areas

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