Functional analysis of microRNA activity in Brugia malayi

Canhui Liu, Denis Voronin, Catherine B. Poole, Saheed Bachu, Matthew B. Rogers, Jingmin Jin, Elodie Ghedin, Sara Lustigman, Larry A. McReynolds, Thomas R. Unnasch

Research output: Contribution to journalArticlepeer-review

Abstract

The complement of the Brugia malayi microRNA-71 was inserted into the 3' untranslated region of a reporter plasmid, resulting in a decrease in reporter activity. Mutation of the seed sequence restored activity. Insertion of the 3' untranslated regions from two algorithm-predicted putative target genes into the reporter resulted in a similar decrease in activity; mutation of the predicted target sequences restored activity. These experiments demonstrate that B. malayi microRNA targets may be predicted using current algorithms and describe a functional assay to confirm predicted targets.

Original languageEnglish (US)
Pages (from-to)579-583
Number of pages5
JournalInternational Journal for Parasitology
Volume45
Issue number9-10
DOIs
StatePublished - Aug 1 2015

Keywords

  • Filariasis
  • Genetic regulation
  • MiRNA
  • Transfection

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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