@article{2e5f694cbb194473be2a8ac19b830ebd,
title = "Functional analysis of microRNA activity in Brugia malayi",
abstract = "The complement of the Brugia malayi microRNA-71 was inserted into the 3' untranslated region of a reporter plasmid, resulting in a decrease in reporter activity. Mutation of the seed sequence restored activity. Insertion of the 3' untranslated regions from two algorithm-predicted putative target genes into the reporter resulted in a similar decrease in activity; mutation of the predicted target sequences restored activity. These experiments demonstrate that B. malayi microRNA targets may be predicted using current algorithms and describe a functional assay to confirm predicted targets.",
keywords = "Filariasis, Genetic regulation, MiRNA, Transfection",
author = "Canhui Liu and Denis Voronin and Poole, {Catherine B.} and Saheed Bachu and Rogers, {Matthew B.} and Jingmin Jin and Elodie Ghedin and Sara Lustigman and McReynolds, {Larry A.} and Unnasch, {Thomas R.}",
note = "Funding Information: Parasite material used in this study was obtained through the Filariasis Research Reagent Resource Center (FR3), Division of Microbiology and infectious Diseases, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health, USA. This work was supported by a grant from the United States NIAID (Project # 1R56AI101372-01A1) to SL, EG and TRU. Publisher Copyright: {\textcopyright} 2015 Australian Society for Parasitology Inc. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",
year = "2015",
month = aug,
day = "1",
doi = "10.1016/j.ijpara.2015.04.004",
language = "English (US)",
volume = "45",
pages = "579--583",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier Limited",
number = "9-10",
}