Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis

Elizabeth A. Winzeler, Daniel D. Shoemaker, Anna Astromoff, Hong Liang, Keith Anderson, Bruno Andre, Rhonda Bangham, Rocio Benito, Jef D. Boeke, Howard Bussey, Angela M. Chu, Carla Connelly, Karen Davis, Fred Dietrich, Sally Whelen Dow, Mohamed El Bakkoury, Françoise Foury, Stephen H. Friend, Erik Gentalen, Guri GiaeverJohannes H. Hegemann, Ted Jones, Michael Laub, Hong Liao, Nicole Liebundguth, David J. Lockhart, Anca Lucau-Danila, Marc Lussier, Nasiha M'Rabet, Patrice Menard, Michael Mittmann, Chai Pai, Corinne Rebischung, Jose L. Revuelta, Linda Riles, Christopher J. Roberts, Petra Ross-MacDonald, Bart Scherens, Michael Snyder, Sharon Sookhai-Mahadeo, Reginald K. Storms, Steeve Véronneau, Marleen Voet, Guido Volckaert, Teresa R. Ward, Robert Wysocki, Grace S. Yen, Kexin Yu, Katja Zimmermann, Peter Philippsen, Mark Johnston, Ronald W. Davis

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The functions of many open reading frames (ORFs) identified in genome- sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.

    Original languageEnglish (US)
    Pages (from-to)901-906
    Number of pages6
    JournalScience
    Volume285
    Issue number5429
    DOIs
    StatePublished - Aug 6 1999

    ASJC Scopus subject areas

    • General

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