Gene-breaking: A new paradigm for human retrotransposon-mediated gene evolution

Sarah J. Wheelan, Yasunori Aizawa, Jeffrey S. Han, Jef D. Boeke

    Research output: Contribution to journalArticle

    Abstract

    The L1 retrotransposon is the most highly successful autonomous retrotransposon in mammals. This prolific genome parasite may on occasion benefit its host through genome rearrangements or adjustments of host gene expression. In examining possible effects of L1 elements on host gene expression, we investigated whether a full-length L1 element inserted in the antisense orientation into an intron of a cellular gene may actually split the gene's transcript into two smaller transcripts: (1) a transcript containing the upstream exons and terminating in the major antisense polyadenylation site (MAPS) of the L1, and (2) a transcript derived from the L1 antisense promoter (ASP) that includes the downstream exons of the gene. Bioinformatic analysis and experimental follow-up provide evidence for this L1 "gene-breaking" hypothesis. We identified three human genes apparently "broken" by L1 elements, as well as 12 more candidate genes. Most of the inserted L1 elements in our 15 candidate genes predate the human/chimp divergence. If indeed split, the transcripts of these genes may in at least one case encode potentially interacting proteins, and in another case may encode novel proteins. Gene-breaking represents a new mechanism through which L1 elements remodel mammalian genomes.

    Original languageEnglish (US)
    Pages (from-to)1073-1078
    Number of pages6
    JournalGenome Research
    Volume15
    Issue number8
    DOIs
    StatePublished - Aug 2005

    ASJC Scopus subject areas

    • Genetics
    • Genetics(clinical)

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