Gene delivery from supercharged coiled-coil protein and cationic lipid hybrid complex

Haresh T. More, Joseph A. Frezzo, Jisen Dai, Seiichi Yamano, Jin K. Montclare

Research output: Contribution to journalArticle

Abstract

A lipoproteoplex comprised of an engineered supercharged coiled-coil protein (CSP) bearing multiple arginines and the cationic lipid formulation FuGENE HD (FG) was developed for effective condensation and delivery of nucleic acids. The CSP was able to maintain helical structure and self-assembly properties while exhibiting binding to plasmid DNA. The ternary CSP·DNA(8:1)·FG lipoproteoplex complex demonstrated enhanced transfection of β-galactosidase DNA into MC3T3-E1 mouse preosteoblasts. The lipoproteoplexes showed significant increases in transfection efficiency when compared to conventional FG and an mTat·FG lipopolyplex with a 6- and 2.5-fold increase in transfection, respectively. The CSP·DNA(8:1)·FG lipoproteoplex assembled into spherical particles with a net positive surface charge, enabling efficient gene delivery. These results support the application of lipoproteoplexes with protein engineered CSP for non-viral gene delivery.

Original languageEnglish (US)
Pages (from-to)7188-7193
Number of pages6
JournalBiomaterials
Volume35
Issue number25
DOIs
StatePublished - Aug 2014

Keywords

  • Cationic lipid
  • Coiled-coil protein
  • Gene delivery
  • Lipoproteoplexes
  • Supercharge

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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